![Immunohistochemistry-Paraffin: Elastase Antibody (CELA3B/1757) [NBP2-53193] - Formalin-fixed, paraffin-embedded Human Pancreas stained with CELA3B Monoclonal Antibody (CELA3B/1757)](http://images.novusbio.com/fullsize/Elastase-Antibody-CELA3B-1757-Immunohistochemistry-Paraffin-NBP2-53193-img0001.jpg "Immunohistochemistry-Paraffin: Elastase Antibody (CELA3B/1757) [NBP2-53193] - Formalin-fixed, paraffin-embedded Human Pancreas stained with CELA3B Monoclonal Antibody (CELA3B/1757)")
| Reactivity | HuSpecies Glossary |
| Applications | ELISA, IHC, MA |
| Clone | CELA3B/1757 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54491) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Recombinant fragment (around aa 82-238) of human Elastase protein (exact sequence is proprietary) (Uniprot: P08861) |
| Localization | Cytoplasmic |
| Marker | Pancreatic Function Marker |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CELA3B |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA. Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
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| Theoretical MW | 17 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
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| Publication using NBP2-53193 | Applications | Species |
|---|---|---|
| Sawaya AP, Stone RC, Mehdizadeh S et al. FOXM1 network in association with TREM1 suppression regulates NET formation in diabetic foot ulcers EMBO reports 2022-08-03 [PMID: 35856334] (ICC, Human) | ICC | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for Elastase Antibody (NBP2-53193)Find related products by research area.
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![Protein Array: Elastase Antibody (CELA3B/1757) [NBP2-53193] - Analysis of Protein Array containing >19,000 full-length human proteins using Elastase Antibody (CELA3B/1757) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Elastase-Antibody-CELA3B-1757-Protein-Array-NBP2-53193-img0003.jpg "Protein Array: Elastase Antibody (CELA3B/1757) [NBP2-53193] - Analysis of Protein Array containing >19,000 full-length human proteins using Elastase Antibody (CELA3B/1757) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.")
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