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EDNRB/Endothelin R Type B Antibody [Unconjugated]

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A172 human glioblastoma cell line was stained with Human EDNRB/Endothelin R Type B Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4496, filled histogram) or isotype control antibody (Catalog # 5‑001‑A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).
A172 human glioblastoma cell line was stained with Human EDNRB/Endothelin R Type B Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4496, filled histogram) or isotype control antibody (Catalog # 5‑001‑A, ...read more
HEK293 human embryonic kidney cell line transfected with either (A) human EDNRB or (B) irrelevant protein and eGFP was stained with Sheep Anti-Human EDNRB Affinity Purified Polyclonal Antibody (Catalog # AF4496) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, CyTOF-ready
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF4496-SP
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EDNRB/Endothelin R Type B Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human EDNRB
Glu27-Lys101
Accession # P24530
Specificity
Detects human EDNRB in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
EDNRB
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
Publications
Read Publication using
AF4496 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EDNRB/Endothelin R Type B Antibody [Unconjugated]

  • ABCDS
  • EDNRB
  • endothelin B receptor
  • Endothelin receptor non-selective type
  • endothelin receptor type B
  • ETB
  • ET-B
  • ETBR
  • ET-BR
  • ETRB
  • HSCR
  • HSCR2
  • WS4A

Background

EDNRB (Endothelin B Receptor) is a member of the beta-family of rhodopsin receptors. It binds endothelin 1, 2, and 3 and is found on endothelial cells where it mediates vasodilation. Mature human EDNRB is a 7-transmembrane glycoprotein that is 416 amino acids (aa) in length. It contains a 75 aa N-terminal extracellular region (aa 27-101), and a 44 aa C-terminal cytoplasmic domain. There are three EDNRB variants that affect aa 27-101. One shows a 90 aa N-terminal extension, a second shows the same 90 aa N-terminal substitution coupled with a deletion of aa 268-398, and a third shows proteolytic cleavage between Arg64-Ser65. Over aa 27-101, human EDNRB shares 67% and 97% aa sequence identity with mouse and canine EDNRB, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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EDNRB/Endothelin R
Type B Antibody [Unco ...
AF4496
EDNRB/Endothelin R Type B Antibody [Unconjugated]
Species: Hu
Applications: Flow, CyTOF-ready

Publications for EDNRB/Endothelin R Type B Antibody (AF4496)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Flow Cytometry.


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Bioinformatics

Gene Symbol EDNRB
Uniprot