Western Blot: DPY30 Antibody [NBP1-91848] - 40 ug total protein per lane, separated by 12% SDS-PAGE and blotted onto a PVDF membrane. DPY30 antibody at 1:200, GAPDH antibody at 1:3000. Secondary antibody at 1:5000. ...read more
Immunocytochemistry/ Immunofluorescence: DPY30 Antibody [NBP1-91848] - Staining of human cell line A-431 shows localization to nucleoplasm & the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: DPY30 Antibody [NBP1-91848] - Staining of human small intestine shows moderate nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: DPY30 Antibody [NBP1-91848] - Staining of human fallopian tube shows moderate to strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: DPY30 Antibody [NBP1-91848] - Staining of human kidney shows moderate to strong nuclear positivity in cells in tubules.
Immunohistochemistry-Paraffin: DPY30 Antibody [NBP1-91848] - Staining of human pancreas shows nuclear positivity.
Immunohistochemistry-Paraffin: DPY30 Antibody [NBP1-91848] - Staining of human prostate shows moderate to strong nuclear positivity in glandular cells.
This antibody was developed against Recombinant Protein corresponding to amino acids: YGLTDNVERIVENEKINAEKSSKQKVDLQSLPTRAYLDQTVVPILLQGLAVLAKERPPNPIEFLASYLLKNKAQFEDRN
Predicted Species
Mouse (99%), Rat (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DPY30
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified
Alternate Names for DPY30 Antibody
dpy-30 homolog (C. elegans)
dpy-30L
Dpy-30-like protein
HDPY-30
protein dpy-30 homolog
Saf19
Background
DPY30 is a component of a Set1-like multiprotein histone methyltransferase complex (Cho et al., 2007 (PubMed17500065)).(supplied by OMIM)
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
These are the main steps of our western blot. The protein concentration was assayed using the BCA protein assay(Thermo REF:23227). For this,40 μg of protein per lanewas separated by 12% SDS-PAGE and electroblotted onto a PVDF membrane. Then, non-specific binding was blocked by incubating with5% non-fat milk in TBST buffer at room temperature for 2 h. PVDF membranes were incubated overnight at 4 °C with primary antibodies which were diluted in TBS-T with 5% non-fat milk as follows: DPY30 (1:200), GAPDH (1:3000). This was followed by incubation with the corresponding secondary antibody(1:5000) at room temperature for 1 h. Finally, antigens were detected using the standard chemical luminescence method (ECL).
Product General Protocols
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FAQs for DPY30 Antibody (NBP1-91848). (Showing 1 - 1 of 1 FAQ).
I had a question regarding your Anti-DPY-30 antibody (NBP1-91848). Online it states that the species reactivity is with human cell lines. When running a BLAST with the antigen sequence you supply, I noticed that it is 99% homologous with mouse and hamster cell lines. I was wondering if you happened to have any support/references of this antibody reacting with these cell lines, particularly in immunocytochemistry/immunofluorescence experiments?
We have unfortunately only tested this antibody in human samples. As the sequences between mouse, hamster and our product are similar, the chance for detection using this antibody are good. If you would like to use this product in your experiment, we can offer you our Innovators Reward Program. In exchange for a review of your experiment on our product page, we will issue you a credit for the purchase price of the antibody.
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