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PTIP Antibody

Images

 
Western Blot: PTIP Antibody [NBP1-88233] - Staining of human liver shows low expression as expected.
Immunohistochemistry-Paraffin: PTIP Antibody [NBP1-88233] - Staining of human testis shows high expression.
Western Blot: PTIP Antibody [NBP1-88233] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted)
Immunohistochemistry-Paraffin: PTIP Antibody [NBP1-88233] - Staining of human lymph node shows strong nuclear positivity in reaction center cells and lymphoid cells outside reaction center.
Orthogonal Strategies: Immunohistochemistry-Paraffin: PTIP Antibody [NBP1-88233] - Staining in human testis and liver tissues using anti-PAXIP1 antibody. Corresponding PAXIP1 RNA-seq data are presented for the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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PTIP Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: EEEEEVENEEQDSQNEGSTDEKSSPASSQEGSPSGDQQFSPKSNTEKSKGELMFDDSSDSSPEKQERNLNWTPAEVPQLAAAKRRLPQGKEPGLINLCANVPPVPGNILPPEVRGNLMAAGQNLQSSERS
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PAXIP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
PTIP Protein (NBP1-88233PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PTIP Antibody

  • CAGF28
  • PACIP1
  • PAX interacting (with transcription-activation domain) protein 1
  • PAX transactivation activation domain-interacting protein
  • PAX transcription activation domain interacting protein 1 like
  • PAX-interacting protein 1
  • PAXIP1LCAGF29
  • protein encoded by CAG trinucleotide repeats
  • PTIPFLJ41049
  • TNRC2

Background

PTIP facilitates ATM-mediated activation of p53 and promotes cellular resistance to ionizing radiation. Tandem BRCT (BRCA1 carboxyl-terminal) domains in PTIP and in BRCA1 serve as phosphoserine or phosphothreonine specific binding modules that recognize substrates phosphorylated by the kinases ATM and ATR in response to gamma irradiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PTIP Antibody (NBP1-88233) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PAXIP1