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Rat Dkk-1 ELISA Kit (Colorimetric)

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ELISA: Rat Dkk-1 ELISA Kit (Colorimetric) [NBP2-82461] - Samples were spiked with high concentrations of Rat Dkk-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Rat Dkk-1 ELISA Kit (Colorimetric) [NBP2-82461] - Samples were spiked with high concentrations of Rat Dkk-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of ...read more
ELISA: Rat Dkk-1 ELISA Kit (Colorimetric) [NBP2-82461] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids
Standard Curve Range
0.31 - 20 ng/mL
Sensitivity
0.19 ng/mL

Order Details

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Rat Dkk-1 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat Dkk-1 in samples. No Significant cross-reactivity or interference between Rat Dkk-1 and analogues was observed.
Standard Curve Range
0.31 - 20 ng/mL
Sensitivity
0.19 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.63
Intra-Assay
CV% < 5.7
Spike Recovery
86-109%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
DKK1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat Dkk-1 ELISA Kit (Colorimetric)

  • dickkopf (Xenopus laevis) homolog 1
  • dickkopf homolog 1 (Xenopus laevis)
  • dickkopf related protein-1
  • Dickkopf-1
  • dickkopf-related protein 1
  • Dkk1
  • Dkk-1
  • hDkk-1
  • SKdickkopf-1 like

Background

DKK1 is a secreted factor that has been linked to the function of osteoblasts. Elevated DKK1 levels in bone marrow plasma and peripheral blood from patients with multiple myeloma correlated with the gene-expression patterns of DKK1 and were associated with the presence of focal bone lesions. The production of DKK1, an inhibitor of osteoblast differentiation, by myeloma cells is associated with the presence of lytic bone lesions in patients with multiple myeloma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Contact Information

Product PDFs

  SDS
  Rat Dkk-1 ELISA Kit (Colorimetric) Manual
  PDF Datasheet
  Request CofA

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Bioinformatics

Gene Symbol DKK1