Dkk-1 Antibody

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy (1, 2). Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Each domain contains ten cysteine residues (1‑3). Mature mouse Dkk-1 is a 40 kDa glycosylated protein that shares 86%, 96%, 83% and 82% amino acid (aa) sequence identity with human, rat, rabbit and bovine Dkk‑1, respectively. It also shares 41% and 36% aa identity with human Dkk-2 and Dkk-4, respectively. Dkk-1 and Dkk-4 are well documented antagonists of the canonical Wnt signaling pathway (1, 2). This pathway is activated by Wnt engagement of a receptor complex composed of the Frizzled proteins and one of two low‑density lipoprotein receptor-related proteins, LRP5 or LRP6 (4). Dkk-1 antagonizes Wnt by forming ternary complexes of LRP5/6 with Kremen1 or Kremen2 (4, 5). Dkk-1/LRP6/Krm2 complex internalization has been shown to downregulate Wnt signaling (4, 5). Dkk-1 is expressed throughout development and antagonizes Wnt-7a during limb development (6, 7). Other sites of expression include developing neurons, hair follicles and the retina of the eye (8, 9). The balance between Wnt signaling and Dkk-1 inhibition is critical for bone formation and homeostasis (10). Insufficient or excess Dkk-1 activity in bone results in increased or decreased bone density, respectively (8, 11). In adults, Dkk-1 is expressed in osteoblasts and osteocytes, and neurons. Cerebral ischemia induces Dkk-1 expression, which contributes to neuronal cell death (12).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: IHC, IHC-Fr, IHC-P, Immunohistochemistry, Neutralization, Western Blot.

Showing Publications 1 - 10 of 10.

Publications using AF1765	Applications	Species
Turkkahraman, H;Flanagan, S;Zhu, T;Akel, N;Marino, S;Ortega-Gonzalez, D;Yuan, X;Bellido, T; Sclerostin antibody corrects periodontal disease in type 2 diabetic mice JCI insight 2024-07-18 [PMID: 39171525] (Immunohistochemistry, Mouse)	Immunohistochemistry	Mouse
Kenzo Ivanovitch, Pablo Soro-Barrio, Probir Chakravarty, Rebecca A. Jones, Donald M. Bell, S. Neda Mousavy Gharavy et al. Ventricular, atrial, and outflow tract heart progenitors arise from spatially and molecularly distinct regions of the primitive streak PLOS Biology 2021-05-17 [PMID: 33999917]
N Kawamura, K Takaoka, H Hamada, AK Hadjantona, GH Sun-Wada, Y Wada Rab7-Mediated Endocytosis Establishes Patterning of Wnt Activity through Inactivation of Dkk Antagonism Cell Rep, 2020-06-09;31(10):107733. 2020-06-09 [PMID: 32521258] (IHC, Mouse)	IHC	Mouse
DL Coutu, KD Kokkaliari, L Kunz, T Schroeder Multicolor quantitative confocal imaging cytometry Nat. Methods, 2017-11-13;15(1):39-46. 2017-11-13 [PMID: 29320487] (IHC, Mouse)	IHC	Mouse
Dongyue Wang, Yajuan Weng, Shuyu Guo, Yuxin Zhang, Tingting Zhou, Mengnan Zhang et al. Platelet-rich plasma inhibits RANKL-induced osteoclast differentiation through activation of Wnt pathway during bone remodeling International Journal of Molecular Medicine 2017-11-16 [PMID: 29207140]
Dickkopf-1 promotes hematopoietic regeneration via direct and niche-mediated mechanisms Nat. Med, 2016-12-05;0(0):. 2016-12-05 [PMID: 27918563] (Neutralization, Mouse)	Neutralization	Mouse
Matrisciano F, Busceti CL, Bucci D, Orlando R, Caruso A, Molinaro G, Cappuccio I, Riozzi B, Gradini R, Motolese M, Caraci F, Copani A, Scaccianoce S, Melchiorri D, Bruno V, Battaglia G, Nicoletti F Induction of the Wnt antagonist Dickkopf-1 is involved in stress-induced hippocampal damage. PLoS ONE, 2011-01-27;6(1):e16447. 2011-01-27 [PMID: 21304589] (IHC-Fr, Western Blot, Mouse)	IHC-Fr, Western Blot	Mouse
Trowbridge JJ, Guezguez B, Moon RT Wnt3a activates dormant c-Kit(-) bone marrow-derived cells with short-term multilineage hematopoietic reconstitution capacity. Stem Cells, 2010-08-01;28(8):1379-89. 2010-08-01 [PMID: 20521329] (Neutralization, Mouse)	Neutralization	Mouse
Heath DJ, Chantry AD, Buckle CH, Coulton L, Shaughnessy JD, Evans HR, Snowden JA, Stover DR, Vanderkerken K, Croucher PI Inhibiting Dickkopf-1 (Dkk1) removes suppression of bone formation and prevents the development of osteolytic bone disease in multiple myeloma. J. Bone Miner. Res., 2009-03-01;24(3):425-36. 2009-03-01 [PMID: 19016584] (IHC-P, Mouse)	IHC-P	Mouse
Diarra D, Stolina M, Polzer K, Zwerina J, Ominsky MS, Dwyer D, Korb A, Smolen J, Hoffmann M, Scheinecker C, van der Heide D, Landewe R, Lacey D, Richards WG, Schett G Dickkopf-1 is a master regulator of joint remodeling. Nat. Med., 2007-01-21;13(2):156-63. 2007-01-21 [PMID: 17237793] (IHC-P, Western Blot, Human, Mouse)	IHC-P, Western Blot	Human, Mouse

Images	Ratings	Applications	Species	Date	Details
	4 reviewed by: Verified Customer	IHC-P	Mouse	01/05/2015	View Summary Application Immunohistochemistry-Paraffin Sample Tested See PMID 20050968 Species Mouse

Array AF1765

• Dkk-1 Antibodies
• Dkk-1 Antibody Pair
• Dkk-1 ELISA Kit
• Dkk-1 Lysate
• Dkk-1 Proteins and Enzymes
• Dkk-1 ELISAs