![Western Blot: Dihydrofolate Reductase/DHFR Antibody [NBP2-16163] - A. 30 ug PC-12 whole cell lysate/extract 12 % SDS-PAGE DHFR antibody dilution: 1:1000](http://images.novusbio.com/fullsize/Dihydrofolate-Reductase-DHFR-Antibody-Western-Blot-NBP2-16163-img0005.jpg "Western Blot: Dihydrofolate Reductase/DHFR Antibody [NBP2-16163] - A. 30 ug PC-12 whole cell lysate/extract 12 % SDS-PAGE DHFR antibody dilution: 1:1000")
| Reactivity | Hu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Dihydrofolate Reductase/DHFR. The exact sequence is proprietary. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | DHFR |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 21 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
![Western Blot RFC1 Antibody [Unconjugated]](https://images.novusbio.com/images2/RFC1_AF6457_Western_Blot_9874.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for Dihydrofolate Reductase/DHFR Antibody (NBP2-16163)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: Dihydrofolate reductase Antibody [NBP2-16163] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Dihydrofolate-reductase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16163-img0003.jpg "Immunocytochemistry/Immunofluorescence: Dihydrofolate reductase Antibody [NBP2-16163] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: Dihydrofolate reductase Antibody [NBP2-16163] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Dihydrofolate-reductase-Antibody-Immunohistochemistry-Paraffin-NBP2-16163-img0002.jpg "Immunohistochemistry-Paraffin: Dihydrofolate reductase Antibody [NBP2-16163] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:500 dilution.")
![Immunoprecipitation: Dihydrofolate Reductase/DHFR Antibody [NBP2-16163] - Immunoprecipitation of DHFR protein from 293T whole cell extracts using 5 ug of DHFR antibody. Western blot analysis was performed using DHFR antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Dihydrofolate-Reductase-DHFR-Antibody-Immunoprecipitation-NBP2-16163-img0008.jpg "Immunoprecipitation: Dihydrofolate Reductase/DHFR Antibody [NBP2-16163] - Immunoprecipitation of DHFR protein from 293T whole cell extracts using 5 ug of DHFR antibody. Western blot analysis was performed using DHFR antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.")
![Western Blot: Dihydrofolate Reductase/DHFR Antibody [NBP2-16163] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Dihydrofolate Reductase/DHFR antibody (NBP2-16163) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-16163_rabbit-polyclonal-dihydrofolate-reductase-dhfr-antibody-910202420122097.jpg "Western Blot: Dihydrofolate Reductase/DHFR Antibody [NBP2-16163] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Dihydrofolate Reductase/DHFR antibody (NBP2-16163) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")