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DIAPH3 Antibody

Images

 
Western Blot: DIAPH3 Antibody [NBP3-12956] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DIAPH3 antibody (NBP3-12956) diluted at 1:500.
Immunohistochemistry-Paraffin: DIAPH3 Antibody [NBP3-12956] - DIAPH3 antibody detects DIAPH3 protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. DIAPH3 ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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DIAPH3 Antibody Summary

Description
Centrifuge briefly prior to opening.
Immunogen
Recombinant protein encompassing a sequence within the center region of human DIAPH3. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DIAPH3
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot

Reactivity Notes

Mouse (87%), Rat (87%), Bovine (82%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for DIAPH3 Antibody

  • DIAP3
  • diaphanous homolog 3 (Drosophila)
  • diaphanous-related formin 3
  • DKFZp434C0931
  • DKFZp686A13178
  • homolog) 3
  • mDia2
  • protein diaphanous homolog 3

Background

DIAPH3 binds to GTP-bound form of Rho and to profilin. Acts in a Rho-dependent manner to recruit profilin to themembrane, where it promotes actin polymerization. It is required for cytokinesis, stress fiber formation, andtranscriptional activation of the serum r

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DIAPH3