![Immunohistochemistry-Paraffin: Desmoglein-3 Antibody (DSG3/2838) [NBP3-07876] - Formalin-fixed, paraffin-embedded human Esophageal Carcinoma stained with Desmoglein-3 Mouse Monoclonal Antibody (DSG3/2838).](http://images.novusbio.com/fullsize/Desmoglein-3-Antibody-DSG3-2838-Immunohistochemistry-Paraffin-NBP3-07876-img0003.jpg "Immunohistochemistry-Paraffin: Desmoglein-3 Antibody (DSG3/2838) [NBP3-07876] - Formalin-fixed, paraffin-embedded human Esophageal Carcinoma stained with Desmoglein-3 Mouse Monoclonal Antibody (DSG3/2838).")
| Reactivity | HuSpecies Glossary |
| Applications | IHC, MA |
| Clone | DSG3/2838 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08430) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Recombinant fragment (around aa 379-491) human Desmoglein-3 protein (exact sequence is proprietary) (Uniprot: P32926) |
| Localization | Cell Surface |
| Marker | Squamous Cell Marker |
| Specificity | Recognizes a protein of 130kDa, identified as Desmoglein-3 (DSG3). This monoclonal antibody is highly specific to Desmoglein-3 and does not cross-react with other members of the Desmoglein-family. DSG3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Research has shown that DSG3 has a very high sensitivity (80%) and specificity (100%) in recognizing squamous cell carcinoma (SqCC). Therefore, DSG3 is considered a very important marker for lung SqCC and can be a useful ancillary marker to separate SqCC from other subtypes of lung cancer. Moreover, studies have shown that DSG3 expression in lung SqCC may indicate a poor prognosis. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | DSG3 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 130 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
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Epithelial-Mesenchymal Transition (EMT) Markers Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a... Read full blog post. |
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![Immunohistochemistry-Paraffin: Desmoglein-3 Antibody (DSG3/2838) [NBP3-07876] - Formalin-fixed, paraffin-embedded human Esophageal Carcinoma stained with Desmoglein-3 Mouse Monoclonal Antibody (DSG3/2838).](http://images.novusbio.com/fullsize/Desmoglein-3-Antibody-DSG3-2838-Immunohistochemistry-Paraffin-NBP3-07876-img0002.jpg "Immunohistochemistry-Paraffin: Desmoglein-3 Antibody (DSG3/2838) [NBP3-07876] - Formalin-fixed, paraffin-embedded human Esophageal Carcinoma stained with Desmoglein-3 Mouse Monoclonal Antibody (DSG3/2838).")
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