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Desmin Antibody - C-terminus

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 (1 Review)
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Western Blot: Desmin Antibody [NBP1-45143] - Analysis of human (A), mouse (B), rat (C) and pig (D) skeletal muscle lysate (35ug protein in RIPA buffer) probed with Goat anti Human desmin antibody (NBP1-45143) at 1.0ugml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence
Western Blot: Desmin Antibody [NBP1-45143] - Analysis of human (A), mouse (B), rat (C) and pig (D) skeletal muscle lysate (35ug protein in RIPA buffer) probed with Goat anti Human desmin antibody (NBP1-45143) at ...read more
Immunohistochemistry-Paraffin: Desmin Antibody [NBP1-45143] - Staining showing strong specific labeling in pericytes around blood vessels in the brain. Antigen retrieval at pH6 (citra) was performed and Desmin was used ...read more
Western Blot: Desmin Antibody [NBP1-45143] - Human skeletal muscle cell lysate probed with Goat anti Human Desmin C-Terminal (NBP1-45143)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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Desmin Antibody - C-terminus Summary

Immunogen
Synthetic peptide sequence C-RDGEVVSEATQQQHE from the C-Terminal region of Desmin (NP_001918.3)
Specificity
Goat anti Human Desmin recognizes an epitope within the C-terminal (CT) region of human.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DES
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA 1:2000
  • Immunohistochemistry 3-6 ug/ml
  • Immunohistochemistry-Paraffin 3-6 ug/ml
  • Western Blot 0.1-0.3 ug/ml
Application Notes
This product requires heat-mediated antigen retrieval prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose. Goat anti Human Desmin detects a band of approximately 55kDa in human skeletal muscle cell lysates.
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-45143 in the following applications:
Publications
Read Publications using
NBP1-45143 in the following applications:

  • WB
    2 publications

Reactivity Notes

Based on sequence similarity this antibody is expected to react with: Dog, Bovine.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Desmin Antibody - C-terminus

  • CMD1I
  • CMD1IFLJ41013
  • CSM1
  • CSM2
  • DES
  • Desmin
  • FLJ12025
  • FLJ39719
  • FLJ41793
  • intermediate filament protein

Background

Desmins belongs to the intermediate filament family, and are class III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z line structures. Defects in Desmin are the cause of desmin related cardio skeletal myopathy (CSM) also known as desmin related myopathy (DRM). CSM is characterized by skeletal muscle weakness associated with cardiac conduction blocks, arrhythmias, restrictive heart failure, and by intracytoplasmic accumulation of desmin reactive deposits in cardiac and skeletal muscle cells. A desmin related myopathy can have a distal onset, it is then known as hereditary distal myopathy (HDM). Defects in Desmin are also the cause of dilated cardiomyopathy type 1I (CMD1I). CMD1I is an autosomal form of dilated cardiomyopathy characterized by ventricular dilatation and impaired systolic function. Antidesmin antibodies are useful in identification of tumours of myogenic origin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Desmin Antibody (NBP1-45143)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(2)
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(1)
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Review for Desmin Antibody (NBP1-45143) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-45143:
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IHC-P
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Desmin NBP1-45143
Enlarge 		5
reviewed by:
Verified Customer
 IHC-P Human 03/01/2019
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedbrain and spinal cord
SpeciesHuman
Lot161206

Comments

CommentsAntigen retrieval at pH6 fro 20 minutes in a steamer; Used at 1:500 concentration. Novared (Vector labs) was used as a chromogen to develop the signal.

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Immunohistochemistry-Paraffin Desmin NBP1-45143
Enlarge
 Verified Customer
03/01/2019
Application: IHC-P
Species: Human
read this review

Bioinformatics

Gene Symbol DES
Entrez
Uniprot