![Immunohistochemistry-Paraffin: Desmin Antibody (DES/3255) [NBP3-13866] - Formalin-fixed, paraffin-embedded cow small intestine stained with Desmin antibody (DES/3255) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Desmin-Antibody-DES-3255-Immunohistochemistry-Paraffin-NBP3-13866-img0007.jpg "Immunohistochemistry-Paraffin: Desmin Antibody (DES/3255) [NBP3-13866] - Formalin-fixed, paraffin-embedded cow small intestine stained with Desmin antibody (DES/3255) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.")
| Reactivity | Hu, Bv, Ca, Eq, FeSpecies Glossary |
| Applications | IHC |
| Clone | DES/3255 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14044) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. |
| Immunogen | Recombinant full-length human desmin protein (Uniprot: P17661 ) |
| Localization | Cytoplasm |
| Marker | Muscle Cell Marker |
| Specificity | Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. IFs are constructed from two-chain -helical coiled-coil molecules arranged on an imperfect helical lattice, and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. Vimentin is an IF general marker of cells originating in the mesenchyme. Vimentin and Desmin, a related class III IF, are both expressed during skeletal muscle development. Desmin, a 469 amino acid protein found near the Z line in sarcomeres, is expressed more frequently in adult differentiated state tissues. Anti-desmin detects cells of normal smooth, skeletal, and cardiac muscles. Antibody reacts with leiomyomas, leiomyosarcoma, rhabdomyomas, rhabdomyosarcoma, and perivascular cells of glomus tumors of the skin. |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | DES |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes |
| Theoretical MW | 52 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Desmin Antibody (NBP3-13866)Find related products by research area.
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Alpha-smooth muscle actin and the modulation of endothelial and epithelial cell biology Actin is essential for a wide range of cell functions, ranging from cell division and chromatin remodeling to vesicle trafficking and maintenance of cellular structure. In fact, mislocalization of actin to cell junctions during development leads t... Read full blog post. |
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Vimentin in Wound Healing Vimentin is a fundamental 10 nm type III intermediate filament (IF) protein found in many mesenchymal and epithelia tissues, tissue culture cells, and developing neuronal and astrocytic precursor cells of the central nervous system. It frequently co-p... Read full blog post. |
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![Immunohistochemistry-Paraffin: Desmin Antibody (DES/3255) [NBP3-13866] - Formalin-fixed, paraffin-embedded human skeletal muscle stained with Desmin antibody (DES/3255) at 2ug/ml. Inset: PBS instead of primary antibody; secondary only negative control.](http://images.novusbio.com/fullsize/Desmin-Antibody-DES-3255-Immunohistochemistry-Paraffin-NBP3-13866-img0001.jpg "Immunohistochemistry-Paraffin: Desmin Antibody (DES/3255) [NBP3-13866] - Formalin-fixed, paraffin-embedded human skeletal muscle stained with Desmin antibody (DES/3255) at 2ug/ml. Inset: PBS instead of primary antibody; secondary only negative control.")
![Immunohistochemistry-Paraffin: Desmin Antibody (DES/3255) [NBP3-13866] - Formalin-fixed, paraffin-embedded human prostate stained with Desmin antibody (DES/3255) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Desmin-Antibody-DES-3255-Immunohistochemistry-Paraffin-NBP3-13866-img0002.jpg "Immunohistochemistry-Paraffin: Desmin Antibody (DES/3255) [NBP3-13866] - Formalin-fixed, paraffin-embedded human prostate stained with Desmin antibody (DES/3255) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.")
![Immunohistochemistry-Paraffin: Desmin Antibody (DES/3255) [NBP3-13866] - Formalin-fixed, paraffin-embedded human cardiac muscle stained with Desmin antibody (DES/3255) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Desmin-Antibody-DES-3255-Immunohistochemistry-Paraffin-NBP3-13866-img0003.jpg "Immunohistochemistry-Paraffin: Desmin Antibody (DES/3255) [NBP3-13866] - Formalin-fixed, paraffin-embedded human cardiac muscle stained with Desmin antibody (DES/3255) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.")
![Immunohistochemistry-Paraffin: Desmin Antibody (DES/3255) [NBP3-13866] - Formalin-fixed, paraffin-embedded cat smooth muscle stained with Desmin antibody (DES/3255) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Desmin-Antibody-DES-3255-Immunohistochemistry-Paraffin-NBP3-13866-img0004.jpg "Immunohistochemistry-Paraffin: Desmin Antibody (DES/3255) [NBP3-13866] - Formalin-fixed, paraffin-embedded cat smooth muscle stained with Desmin antibody (DES/3255) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.")
![Immunohistochemistry-Paraffin: Desmin Antibody (DES/3255) [NBP3-13866] - Formalin-fixed, paraffin-embedded dog smooth muscle stained with Desmin antibody (DES/3255) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Desmin-Antibody-DES-3255-Immunohistochemistry-Paraffin-NBP3-13866-img0005.jpg "Immunohistochemistry-Paraffin: Desmin Antibody (DES/3255) [NBP3-13866] - Formalin-fixed, paraffin-embedded dog smooth muscle stained with Desmin antibody (DES/3255) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.")
![Immunohistochemistry-Paraffin: Desmin Antibody (DES/3255) [NBP3-13866] - Formalin-fixed, paraffin-embedded horse smooth muscle stained with Desmin antibody (DES/3255) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Desmin-Antibody-DES-3255-Immunohistochemistry-Paraffin-NBP3-13866-img0006.jpg "Immunohistochemistry-Paraffin: Desmin Antibody (DES/3255) [NBP3-13866] - Formalin-fixed, paraffin-embedded horse smooth muscle stained with Desmin antibody (DES/3255) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.")
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