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Recombinant Human delta-Sarcoglycan His Protein

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SDS-Page: Recombinant Human delta-Sarcoglycan His Protein [NBP2-23452] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

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Recombinant Human delta-Sarcoglycan His Protein Summary

Description
A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 57-289 of Human delta-Sarcoglycan

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH RSHMKVMNFT IDGMGNLRIT EKGLKLEGDS EFLQPLYAKE IQSRPGNALY FKSARNVTVN ILNDQTKVLT QLITGPKAVE AYGKKFEVKT VSGKLLFSAD NNEVVVGAER LRVLGAEGTV FPKSIETPNV RADPFKELRL ESPTRSLVME APKGVEINAE AGNMEATCRT ELRLESKDGE IKLDAAKIRL PRLPHGSYTP TGTRQKVFEI CVCANGRLFL SQAGAGSTCQ INTSVCL

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
SGCD
Purity
>85%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • SDS-Page
Application Notes
Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
Theoretical MW
28.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>85%, by SDS-PAGE

Alternate Names for Recombinant Human delta-Sarcoglycan His Protein

  • 35 kDa dystrophin-associated glycoprotein
  • 35DAG
  • 35kD dystrophin-associated glycoprotein
  • CMD1L
  • CMD1LSG-delta
  • DAGD
  • deltaSarcoglycan
  • delta-Sarcoglycan
  • delta-SG
  • dystrophin associated glycoprotein, delta sarcoglycan
  • LGMD2F
  • MGC22567
  • placental delta sarcoglycan
  • sarcoglycan, delta (35kD dystrophin-associated glycoprotein)
  • sarcoglycan, delta (35kDa dystrophin-associated glycoprotein)
  • SGCD
  • SGCDP
  • SGD

Background

SGCD is one of the four known components of the sarcoglycan complex, which is a subcomplex of the dystrophin-glycoprotein complex (DGC). DGC forms a link between the F-actin cytoskeleton and the extracellular matrix. This protein is expressed most abundantly in skeletal and cardiac muscle. The mutations in this gene have been associated with autosomal recessive limb-girdle muscular dystrophy and dilated cardiomyopathy. Alternatively spliced transcript variants encoding distinct isoforms have been observed. In melanocytic cells SGCD gene expression may be regulated by MITF. Recombinant human SGCD protein, fused to His-tag at N-terminus, was expressed in E.coli.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol SGCD