![Western Blot: DEC-205/CD205 Antibody (JB87-35) [NBP2-75467] - Analysis of LY75 on THP-1 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/DEC-205-CD205-Antibody-JB87-35-Western-Blot-NBP2-75467-img0005.jpg "Western Blot: DEC-205/CD205 Antibody (JB87-35) [NBP2-75467] - Analysis of LY75 on THP-1 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, IHC, IP |
| Clone | JB87-35 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Recombinant protein within Human DEC-205/CD205 aa 1,560-1,722 / 1,722. (SwissProt: O60449 Human; SwissProt: Q60767 Mouse) |
| Localization | Membrane. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | LY75 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 198 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for DEC-205/CD205 Antibody (NBP2-75467)Find related products by research area.
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| Gene Symbol | LY75 |
![Immunohistochemistry-Paraffin: DEC-205/CD205 Antibody (JB87-35) [NBP2-75467] - Analysis of paraffin-embedded mouse colon tissue using anti-LY75 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/DEC-205-CD205-Antibody-JB87-35-Immunohistochemistry-Paraffin-NBP2-75467-img0004.jpg "Immunohistochemistry-Paraffin: DEC-205/CD205 Antibody (JB87-35) [NBP2-75467] - Analysis of paraffin-embedded mouse colon tissue using anti-LY75 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: DEC-205/CD205 Antibody (JB87-35) [NBP2-75467] - Analysis of paraffin-embedded human tonsil tissue using anti-LY75 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/DEC-205-CD205-Antibody-JB87-35-Immunohistochemistry-Paraffin-NBP2-75467-img0001.jpg "Immunohistochemistry-Paraffin: DEC-205/CD205 Antibody (JB87-35) [NBP2-75467] - Analysis of paraffin-embedded human tonsil tissue using anti-LY75 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: DEC-205/CD205 Antibody (JB87-35) [NBP2-75467] - Analysis of paraffin-embedded human colon cancer tissue using anti-LY75 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/DEC-205-CD205-Antibody-JB87-35-Immunohistochemistry-Paraffin-NBP2-75467-img0002.jpg "Immunohistochemistry-Paraffin: DEC-205/CD205 Antibody (JB87-35) [NBP2-75467] - Analysis of paraffin-embedded human colon cancer tissue using anti-LY75 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: DEC-205/CD205 Antibody (JB87-35) [NBP2-75467] - Analysis of paraffin-embedded human kidney tissue using anti-LY75 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/DEC-205-CD205-Antibody-JB87-35-Immunohistochemistry-Paraffin-NBP2-75467-img0003.jpg "Immunohistochemistry-Paraffin: DEC-205/CD205 Antibody (JB87-35) [NBP2-75467] - Analysis of paraffin-embedded human kidney tissue using anti-LY75 antibody. Counter stained with hematoxylin.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")