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Recombinant Human DDIT4 His Protein

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SDS-Page: Recombinant Human DDIT4 Protein [NBP1-50976] - 15 % SDS-PAGE (3ug)
SDS-Page: Recombinant Human DDIT4 Protein [NBP1-50976] - 15 % SDS-PAGE (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.25 mg/ml

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Recombinant Human DDIT4 His Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-232 of Human DDIT4

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MPSLWDRFSS SSTSSSPSSL PRTPTPDRPP RSAWGSATRE EGFDRSTSLE SSDCESLDSS NSGFGPEEDT AYLDGVSLPD FELLSDPEDE HLCANLMQLL QESLAQARLG SRRPARLLMP SQLVSQVGKE LLRLAYSEPC GLRGALLDVC VEQGKSCHSV GQLALDPSLV PTFQLTLVLR LDSRLWPKIQ GLFSSANSPF LPGFSQSLTL STGFRVIKKK LYSSEQLLIE EC
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
DDIT4
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
27.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH8.0), 0.2 M NaCl, 5mM DTT, 1 mM EDTA, 30% glycerol
Preservative
No Preservative
Concentration
0.25 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human DDIT4 His Protein

  • Dig2
  • DNA-damage-inducible transcript 4
  • FLJ20500
  • HIF-1 responsive RTP801 (RTP801)
  • Protein regulated in development and DNA damage response 1
  • REDD-1DNA damage-inducible transcript 4 protein
  • REDD1HIF-1 responsive protein RTP801
  • RTP801RP11-442H21.1

Background

DDIT4, also known as Dig2 or REDD1, is thought to have function in the regulation of reactive oxygen species. In response to stress due to DNA damage and glucocorticoid treatment, DDIT4 is upregulated at the transcriptional level. DDIT4 negatively regulates the mammalian target of Rapamycin, a serine/threonine kinase often referred to as mTOR. It is crucial in the coupling of extra- and intracellular cues to mTOR regulation. Recombinant human DDIT4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol DDIT4