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DAZL Antibody

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Western Blot: DAZL Antibody [NBP1-85307] - Analysis in control (vector only transfected HEK293T lysate) and DAZL over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: DAZL Antibody [NBP1-85307] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
Orthogonal Strategies: Immunohistochemistry-Paraffin: DAZL Antibody [NBP1-85307] - Analysis in human testis and endometrium tissues. Corresponding DAZL RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: DAZL Antibody [NBP1-85307] - Staining of human cerebral cortex shows no positivity in neurons as expected.
Independent Antibodies: Immunohistochemistry-Paraffin: DAZL Antibody [NBP1-85307] - Staining of human cerebral cortex, endometrium, prostate and testis using Anti-DAZL antibody NBP1-85307 (A) shows similar ...read more
Immunohistochemistry-Paraffin: DAZL Antibody [NBP1-85307] - Staining of human endometrium shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: DAZL Antibody [NBP1-85307] - Staining of human prostate shows no positivity in glandular cells as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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DAZL Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: PTYPNSPVQVITGYQLPVYNYQMPPQWPVGEQRSYVVPPAYSAVNYHCNEVDPGAEVVPNECS
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DAZL
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:10-1:20
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
DAZL Protein (NBP1-85307PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Rat (84%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for DAZL Antibody

  • DAZHSPGY-like-autosomal
  • DAZL1DAZ-like autosomal
  • DAZLA
  • Deleted in azoospermia-like 1
  • deleted in azoospermia-like
  • germline specific RNA binding protein
  • MGC26406
  • spermatogenesis gene on the Y-like autosomal
  • SPGYLADAZ homolog

Background

DAZL is a RNA-binding protein, which is essential for gametogenesis. Plays a central role during spermatogenesis. May act by binding to the 3'-UTR of mRNA and thereby regulating the translation of key transcripts

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol DAZL