Recombinant Human DAP1 His Protein Summary
Description |
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-102 of Human DAP1 Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSSPPEG KLETKAGHPP AVKAGGMRIV QKHPHTGDTK EEKDKDDQEW ESPSPPKPTV FISGVIARGD KDFPPAAAQV AHQKPHASMD KHPSPRTQHI QQPRK |
Source |
E. coli |
Protein/Peptide Type |
Recombinant Protein |
Gene |
DAP |
Purity |
>80%, by SDS-PAGE |
Applications/Dilutions
Dilutions |
|
Theoretical MW |
13.6 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 10% glycerol, 1 mM DTT |
Preservative |
No Preservative |
Concentration |
0.25 mg/ml |
Purity |
>80%, by SDS-PAGE |
Alternate Names for Recombinant Human DAP1 His Protein
Background
Death associated protein 1 (DAP-1) is a 15-kDa protein that functions as a positive mediator of cell death initiated by interferon (IFN-) gamma (1,2). DAP-1 is proline rich and possesses one SH3 binding motif, as well as several consensus protein kinase phosphorylation sites (1). It is localized to the cytoplasm, but the detailed mechanism of its pro-apoptotic function is unclear. DAP-1 and DAP kinase, a unique, 160-kDa calmodulin-dependent serine/threonine kinase, are both unique candidates for positive mediators of programmed cell death induced by IFN-gamma (3).
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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