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Cytokeratin 19 Antibody (KRT19/799) [CoraFluor™ 1]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready, ICC/IF
Clone
KRT19/799
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-47945AF350
Alexa Fluor 405
NBP2-47945AF405
Alexa Fluor 488
NBP2-47945AF488
Alexa Fluor 532
NBP2-47945AF532
Alexa Fluor 594
NBP2-47945AF594
Alexa Fluor 647
NBP2-47945AF647
Alexa Fluor 700
NBP2-47945AF700
Alexa Fluor 750
NBP2-47945AF750
Allophycocyanin
NBP2-47945APC
Biotin
NBP2-47945B
DyLight 350
NBP2-47945UV
DyLight 405
NBP2-47945V
DyLight 488
NBP2-47945G
DyLight 550
NBP2-47945R
DyLight 594
NBP2-47945DL594
DyLight 650
NBP2-47945C
DyLight 680
NBP2-47945FR
DyLight 755
NBP2-47945IR
FITC
NBP2-47945F
HRP
NBP2-47945H
Janelia Fluor 525
NBP2-47945JF525
Janelia Fluor 549
NBP2-47945JF549
Janelia Fluor 646
NBP2-47945JF646
Janelia Fluor 669
NBP2-47945JF669
PE
NBP2-47945PE
PerCP
NBP2-47945PCP
Unconjugated
NBP2-44826-0.1mg
mFluor Violet 450 SE
NBP2-47945MFV450
mFluor Violet 500 SE
NBP2-47945MFV500
mFluor Violet 610 SE
NBP2-47945MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-44826-0.1mg
Azide and BSA Free
NBP2-47945-0.1mg
IHC-Prediluted
NBP2-48086
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Cytokeratin 19 Antibody (KRT19/799) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Recombinant human Cytokeratin 19 protein (Uniprot: P08727)
Localization
Cytoplasmic
Marker
Pancreatic Stem Cell Marker
Specificity
Recognizes a protein of 40kDa, identified as cytokeratin-19 (CK19), which is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50%-60% of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.
Isotype
IgG2a Lambda
Clonality
Monoclonal
Host
Mouse
Gene
KRT19
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Cytokeratin 19 Antibody (KRT19/799) [CoraFluor™ 1]

  • 40-kDa keratin intermediate filament
  • CK19
  • CK-19
  • Cytokeratin 19
  • EndoC
  • K19
  • K1CS
  • keratin 19
  • keratin, type I cytoskeletal 19
  • keratin, type I, 40-kd
  • keratin-19
  • Krt19
  • MGC15366

Background

Cytokeratin 19 (also known as Keratin, type I cytoskeletal 19) belongs to the keratin family which consists of fibrous proteins that make up the framework of epithelial cells. Cytokeratin 19 plays a role in the organization of myofibers. This protein is known to have interactions with ZNF638, FANCG, FAM107A, KRT15 and EXOC8. Cytokeratin 19 has been studied in relation to several disorders and diseases including chordoma, adenoma, hamartoma, adenoiditis, carcinoma, and liver disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol KRT19