| Reactivity | HuSpecies Glossary |
| Applications | WB, IP |
| Clone | 201805 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant human Cystatin S Ser21-Ala141 Accession # P01036 |
| Specificity | Detects human Cystatin S in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) Cystatin SA is observed and no cross-reactivity with rhCystatin D, E, SN, rhFetuin A, rhFetuin B, or rhHPRG (Histidine-Proline-Rich Glycoprotein) is observed. In Western blots, approximately 15% cross‑reactivity with rhCystatins SA and SN (under reducing conditions only) is observed and no cross‑reactivity with rhCystatin A, B, C, D, E/M, rhFetuin A, rhFetuin B, or rhHPRG is observed. |
| Source | N/A |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CST4 |
| Purity Statement | Protein A or G purified from hybridoma culture supernatant |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Publications |
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| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Reconstitution Instructions | Reconstitute at 0.5 mg/mL in sterile PBS. |
Cystatin S is a member of family 2 of the Cystatin superfamily (1). Like Cystatins SA and SN, it is produced by the salivary gland and secreted largely in the submandibular/sublingual saliva (2). Unlike Cystatins SA and SN, Cystatin S is not a potent inhibitor of cysteine proteases such as papain and some cathepsins (3). Cystatin S is partially phosphorylated at Ser21 and Ser23 in saliva (4). The functions of Cystatin S are largely unknown. However, it is able to bind more calcium and bind more rapidly to carbonated apatite than cystatins SA or SN, indicating that Cystatin S may be involved in the mineral balance of the tooth (3).
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| Publication using MAB1296 | Applications | Species |
|---|---|---|
| Ito T, Komiya-Ito A, Okuda K, Minaguchi K, Saitoh E, Yamada S, Kato T Murine monoclonal antibody which can distinguish cystatins SA1 and SA2. Mol. Immunol., 2005-01-08;42(10):1259-63. 2005-01-08 [PMID: 15829315] (ELISA Development, Western Blot, Human) | ELISA Development, Western Blot | Human |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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