![SDS-Page: Recombinant Human Cyclophilin-F Protein [NBC1-18533] - Cyclophilin F(PPIF), 21 kDa (198aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE](http://images.novusbio.com/fullsize/Recombinant-Human-Cyclophilin-F-Protein-SDS-Page-NBC1-18533-img0002.jpg "SDS-Page: Recombinant Human Cyclophilin-F Protein [NBC1-18533] - Cyclophilin F(PPIF), 21 kDa (198aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IP, PAGE, Bioactivity |
| Concentration | 1 mg/ml |
| Description | A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 30-207 of Human Cyclophilin-F Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MCSKGSGDPS SSSSSGNPLV YLDVDANGKP LGRVVLELKA DVVPKTAENF RALCTGEKGF GYKGSTFHRV IPSFMCQAGD FTNHNGTGGK SIYGSRFPDE NFTLKHVGPG VLSMANAGPN TNGSQFFICT IKTDWLDGKH VVFGHVKEGM DVVKKIESFG SKSGRTSKKI VITDCGQLS |
| Details of Functionality | Specific activity is > 900 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin. |
| Source | E. coli |
| Protein/Peptide Type | Recombinant Protein |
| Gene | PPIF |
| Purity | >95%, by SDS-PAGE |
| Endotoxin Note | < 1.0 EU per 1ug of protein (determined by LAL method) |
| Dilutions |
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| Application Notes | Use in immunoprecipitation, and western blot reported in scientific literature (PMID 26387735) |
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| Theoretical MW | 21.1 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 20 mM Tris-HCl buffer (pH 7.5), 1 mM DTT, 10% glycerol |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | >95%, by SDS-PAGE |
| Publication using NBC1-18533 | Applications | Species |
|---|---|---|
| Shanmughapriya S, Rajan S, Hoffman NE et al. SPG7 Is an Essential and Conserved Component of the Mitochondrial Permeability Transition Pore. Cell Press 2015-10-01 [PMID: 26387735] (WB, IP, Human, Mouse) | WB, IP | Human, Mouse |
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| Gene Symbol | PPIF |
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