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CXCL8/IL-8 Antibody (6217) [Phycoerythrin]

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Human peripheral blood monocytes treated with LPS were stained with Mouse Anti-Human CXCL8/IL-8 PE-conjugated Monoclonal Antibody (Catalog # IC208P) and Anti-Human CD14 Fluorescein-conjugated Antibody (panel A). Inhibition of IC208P staining by the addition of excess Recombinant Human IL-8 (Catalog # 208-IL) is shown in panel B. Quadrant markers were set based on control antibody staining (Catalog # IC002G). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Human peripheral blood monocytes treated with LPS were stained with Mouse Anti-Human CXCL8/IL-8 PE-conjugated Monoclonal Antibody (Catalog # IC208P) and Anti-Human CD14 Fluorescein-conjugated Antibody (panel A). ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
6217
Clonality
Monoclonal
Host
Mouse
Conjugate
Phycoerythrin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC208U-100UG
Alexa Fluor 405
IC208V-100UG
Alexa Fluor 488
IC208G-100UG
Alexa Fluor 532
FAB208X
Alexa Fluor 594
IC208T-100UG
Alexa Fluor 647
IC208R-100UG
Alexa Fluor 700
IC208N-100UG
Alexa Fluor 750
IC208S-100UG
Allophycocyanin
FAB208A
Biotin
FAB208B
CoraFluor 1
FAB208CL1
DyLight 350
FAB208D
DyLight 405
FAB208E
DyLight 488
FAB208K
DyLight 550
FAB208L
DyLight 594
FAB208M
DyLight 650
FAB208W
DyLight 680
FAB208Y
DyLight 755
FAB208Z
Fluorescein
IC208F
HRP
FAB208H
Janelia Fluor 525
FAB208JF525
Janelia Fluor 549
FAB208I
Janelia Fluor 585
FAB208JF585
Janelia Fluor 635
FAB208JF635
Janelia Fluor 646
FAB208J
Janelia Fluor 669
FAB208JF669
PerCP
FAB208C
Unconjugated
MAB208-100
mFluor Violet 450 SE
FAB208MFV450
mFluor Violet 500 SE
FAB208MFV500
mFluor Violet 610 SE
FAB208MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB208-100
Carrier Free
IC208F
Carrier Free
MAB208-SP
Carrier Free
MAB208-100
Carrier Free
MAB208-500
Carrier Free
IC208V-100UG
Carrier Free
IC208U-100UG
Carrier Free
IC208T-100UG
Carrier Free
IC208S-100UG
Carrier Free
IC208R-100UG
Carrier Free
IC208N-100UG
Carrier Free
IC208G-100UG
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CXCL8/IL-8 Antibody (6217) [Phycoerythrin] Summary

Immunogen
E. coli-derived recombinant human CXCL8/IL‑8
Ser28-Ser99
Accession # P10145
Specificity
Detects human CXCL8/IL‑8 in ELISAs and Western blots. In Western blots, this antibody shows 100% cross-reactivity with recombinant porcine CXCL8/IL‑8 and no cross-reactivity with rrCXCL3/CINC-2 alpha .
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IL8
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells
Publications
Read Publications using
IC208P in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL8/IL-8 Antibody (6217) [Phycoerythrin]

  • 3-10C
  • AMCF-I
  • C-X-C motif chemokine 8
  • CXCL8
  • CXCL8SCYB8
  • Emoctakin
  • GCP1
  • GCP-1TSG-1
  • IL8
  • IL-8
  • interleukin 8
  • K60
  • LAI
  • LECT
  • LUCT
  • LYNAP
  • MDNCF
  • MDNCFb-ENAP
  • member 8
  • MONAP
  • MONAPGCP1
  • NAF
  • NAP1
  • NAP-1NAP1
  • NCF
  • Neutrophil-activating protein 1
  • Protein 3-10C
  • T cell chemotactic factor
  • T-cell chemotactic factor
  • TCF
  • TSG1

Background

CXCL8, also known as IL-8, is a CXC chemokine that is a potent chemoattractant for neutrophils and has a wide range of other pro-inflammatory effects.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CXCL8/IL-8 Antibody (IC208P)(4)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Flow Cytometry.


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Flow Cytometry
(4)
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Human
(4)
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Bioinformatics

Gene Symbol IL8
Uniprot