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CXCL8/IL-8 Antibody (6217) [DyLight 550]

 
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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, B/N, CyTOF-ready, ELISA, ELISA(Cap), ELISA(Det), Flow, ICC/IF
Clone
6217
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 550

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC208U-100UG
Alexa Fluor 405
IC208V-100UG
Alexa Fluor 488
IC208G-100UG
Alexa Fluor 532
FAB208X
Alexa Fluor 594
IC208T-100UG
Alexa Fluor 647
IC208R-100UG
Alexa Fluor 700
IC208N-100UG
Alexa Fluor 750
IC208S-100UG
Allophycocyanin
FAB208A
Biotin
FAB208B
CoraFluor 1
FAB208CL1
DyLight 350
FAB208D
DyLight 405
FAB208E
DyLight 488
FAB208K
DyLight 594
FAB208M
DyLight 650
FAB208W
DyLight 680
FAB208Y
DyLight 755
FAB208Z
Fluorescein
IC208F
HRP
FAB208H
Janelia Fluor 525
FAB208JF525
Janelia Fluor 549
FAB208I
Janelia Fluor 585
FAB208JF585
Janelia Fluor 635
FAB208JF635
Janelia Fluor 646
FAB208J
Janelia Fluor 669
FAB208JF669
PerCP
FAB208C
Phycoerythrin
IC208P
Unconjugated
MAB208-100
mFluor Violet 450 SE
FAB208MFV450
mFluor Violet 500 SE
FAB208MFV500
mFluor Violet 610 SE
FAB208MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB208-100
Carrier Free
IC208F
Carrier Free
IC208P
Carrier Free
MAB208-SP
Carrier Free
MAB208-100
Carrier Free
MAB208-500
Carrier Free
IC208V-100UG
Carrier Free
IC208U-100UG
Carrier Free
IC208T-100UG
Carrier Free
IC208S-100UG
Carrier Free
IC208R-100UG
Carrier Free
IC208N-100UG
Carrier Free
IC208G-100UG
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CXCL8/IL-8 Antibody (6217) [DyLight 550] Summary

Immunogen
E. coli-derived recombinant human IL-8/CXCL8
Ser28-Ser99
Accession # P10145
Specificity
Detects human IL-8/CXCL8 in ELISAs and Western blots. In Western blots, 100% cross-reactivity with recombinant porcine IL-8/CXCL8 and no cross-reactivity with recombinant rat CXCL3/CINC-2 alpha is observed.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IL8
Purity
Protein A or G purified from hybridoma culture supernatant
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair)
  • ELISA Detection (Matched Antibody Pair)
  • ELISA Standard (Matched Pair)
  • Immunocytochemistry
  • Intracellular Staining by Flow Cytometry
  • Neutralization
  • Simple Western
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified from hybridoma culture supernatant

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for CXCL8/IL-8 Antibody (6217) [DyLight 550]

  • 3-10C
  • AMCF-I
  • C-X-C motif chemokine 8
  • CXCL8
  • CXCL8SCYB8
  • Emoctakin
  • GCP1
  • GCP-1TSG-1
  • IL8
  • IL-8
  • interleukin 8
  • K60
  • LAI
  • LECT
  • LUCT
  • LYNAP
  • MDNCF
  • MDNCFb-ENAP
  • member 8
  • MONAP
  • MONAPGCP1
  • NAF
  • NAP1
  • NAP-1NAP1
  • NCF
  • Neutrophil-activating protein 1
  • Protein 3-10C
  • T cell chemotactic factor
  • T-cell chemotactic factor
  • TCF
  • TSG1

Background

IL8 is a member of the CXC chemokine family. This family of small basic heparan-binding proteins are proinflammatory and primarily mediate the activation and migration of neutrophils into tissue from peripheral blood. This chemokine is one of the major mediators of the inflammatory response and is secreted by several cell types in response to an inflammatory stimulus. It functions as a chemoattractant, and is also a potent angiogenic factor. IL8 attracts neutrophils, basophils, and T-cells, but not monocytes. Cystic fibrosis (CF) is characterized by severe lung inflammation. The inflammatory process is believed to be caused by massive overproduction of the proinflammatory protein IL8, and the high levels of IL8 in the CF lung are therefore believed to be the central mechanism behind CF lung pathophysiology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol IL8