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Recombinant Bovine CXCL4/PF4 Protein

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Product Details

Summary
Reactivity BvSpecies Glossary
Applications Bioactivity
Concentration
LYOPH

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Recombinant Bovine CXCL4/PF4 Protein Summary

Description
A single non-glycosylated polypeptide chain containing 88 amino acids corresponding to CXCL4/PF4 Source: E. coli

Uniprot ID: P02777

Amino Acid Sequence: ESSFPATFVP LPADSEGGED EDLQCVCLKT TSGINPRHIS SLEVIGAGTH CPSPQLLATK KTGRKICLDQ QRPLYKKILK KLLDGDES
Details of Functionality
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration of 10-100ng/ml.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
PF4
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Note
Less than 0.1 EU/ug of CXCL4/PF4 as determined by LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
9.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, 500 mM NaCl, pH 7.0.
Preservative
No Preservative
Concentration
LYOPH
Purity
>95%, by SDS-PAGE and HPLC
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Alternate Names for Recombinant Bovine CXCL4/PF4 Protein

  • chemokine (C-X-C motif) ligand 4
  • C-X-C motif chemokine 4
  • CXCL4
  • CXCL4iroplact
  • Iroplact
  • MGC138298
  • Oncostatin-A
  • PF4
  • PF-4
  • platelet factor 4
  • SCYB4oncostatin-A

Background

Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair (Eisman et al., 1990 [PubMed 1695112]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol PF4