Creatine Kinase BB Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: Creatine Kinase BB Antibody [NBP1-84460] - Analysis in human cerebral cortex and liver tissues. Corresponding Creatine Kinase BB RNA-seq data are presented ...read more
Immunocytochemistry/ Immunofluorescence: Creatine Kinase BB Antibody [NBP1-84460] - Immunofluorescent staining of human cell line HEK 293 shows localization to cytosol.
Immunohistochemistry-Paraffin: Creatine Kinase BB Antibody [NBP1-84460] - Staining of human stomach shows strong cytoplasmic positivity in parietal cells.
Immunohistochemistry-Paraffin: Creatine Kinase BB Antibody [NBP1-84460] - Staining of human cerebral cortex shows weak to moderate cytoplasmic positivity in astrocytes.
Immunohistochemistry-Paraffin: Creatine Kinase BB Antibody [NBP1-84460] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: Creatine Kinase BB Antibody [NBP1-84460] - Staining of human rectum shows moderate cytoplasmic positivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

Creatine Kinase BB Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: VISMQKGGNMKEVFTRFCTGLTQIETLFKSKDYEFMWNPHLGYILTCPSNLGTGLRAGVHIKLPNLGKHEKFSEVLKRLRLQKRGTGGVDTAAVGGVFDVSNADRLGFSEVELVQMVVDGVKLLIEMEQRLEQGQAIDDLMPAQK
Predicted Species
Mouse (99%), Rat (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CKB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot Reported in scientific literature (PMID: 26460485)
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Creatine Kinase BB Recombinant Protein Antigen (NBP1-84460PEP)
Publications
Read Publications using
NBP1-84460 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Creatine Kinase BB Antibody

  • B-CK
  • CKB
  • Creatine Kinase BB
  • Creatine Kinase Isoenzyme

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Creatine Kinase BB Antibody (NBP1-84460)(4)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Creatine Kinase BB Antibody (NBP1-84460) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CKB
Entrez
Uniprot