Coronin-1a Antibody (OTI1A5)

Images

 
Western Blot: Coronin-1a Antibody (1A5) [NBP2-02779] Analysis of extracts (35ug) from 9 different cell lines by using anti-Coronin-1a monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Coronin-1a Antibody (1A5) [NBP2-02779] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Coronin-1a.
Flow Cytometry: Coronin-1a Antibody (1A5) [NBP2-02779] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Coronin-1a antibody, and then ...read more
Western Blot: Coronin-1a Antibody (1A5) [NBP2-02779] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Coronin-1a (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Flow Cytometry: Coronin-1a Antibody (1A5) [NBP2-02779] - Analysis of Hela cells, using anti-Coronin-1a antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: Coronin-1a Antibody (1A5) [NBP2-02779] - Analysis of Jurkat cells, using anti-Coronin-1a antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF
Clone
OTI1A5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Coronin-1a Antibody (OTI1A5) Summary

Immunogen
Full length human recombinant protein of human CORO1A(NP_009005) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CORO1A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:500-2000
Theoretical MW
50.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Coronin-1a Antibody (OTI1A5)

  • CLIPINA
  • clipin-A
  • CORO1
  • coronin, actin binding protein, 1A
  • coronin, actin-binding protein, 1A
  • coronin-1
  • coronin-1A
  • Coronin-like protein A
  • Coronin-like protein p57
  • FLJ41407
  • HCORO1
  • MGC117380
  • p57
  • TACOCLABP
  • Tryptophan aspartate-containing coat protein

Background

This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Alternative splicing results in multiple transcript variants. A related pseudogene has been defined on chromosome 16. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Coronin-1a Antibody (NBP2-02779) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CORO1A
Entrez