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Equine Collagen II ELISA Kit (Colorimetric)

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Product Details

Summary
Reactivity EqSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
Standard Curve Range
123.5 - 10000 pg/mL
Sensitivity
48.3 pg/mL

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Equine Collagen II ELISA Kit (Colorimetric) Summary

Description
Assay time: 2 hours
Standard Curve Range
123.5 - 10000 pg/mL
Sensitivity
48.3 pg/mL
Assay Type
Competitive ELISA
Inter-Assay
%CV < 12
Intra-Assay
%CV < 10
Spike Recovery
81 - 96%
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
COL2A1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Assay Diluent A
  2. Assay Diluent B
  3. Detection Reagent A
  4. Detection Reagent B
  5. Instruction manual
  6. Plate sealer for 96 wells
  7. Pre-coated ready to use 96-well strip plate
  8. Standard Diluent
  9. Standard
  10. Stop Solution
  11. TMB Substrate
  12. Wash Buffer (30 x concentrate)

Alternate Names for Equine Collagen II ELISA Kit (Colorimetric)

  • Alpha-1 type II collagen
  • ANFH
  • AOM
  • cartilage collagen
  • Chondrocalcin
  • COL11A3
  • COL2A1
  • Collagen 2
  • collagen alpha-1(II) chain
  • Collagen II
  • collagen II, alpha-1 polypeptide
  • collagen type 2
  • collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphysealdysplasia, congenital)
  • collagen, type II, alpha 1
  • Collagen-2
  • MGC131516
  • SEDC

Background

The extensive family of COL gene products (collagens) is composed of several chain types, including fibril-forming interstitial collagens (types I, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue, such as tendons and cartilage. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Several collagens also play a role in cell adhesion, important for maintaining normal tissue architecture and function. In cartilage, Collagen Type II constitutes the bulk of the fibril. Sensitization with Collagen Type II induces an erosive polyarthritis in rats, mice and higher primates which can resemble rheumatoid arthritis and relapsing polychrondritis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol COL2A1