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Human Collagen II ELISA Kit (Chemiluminescence)

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ELISA: Human Collagen II ELISA Kit (Chemiluminescence) [NBP2-75852] - Samples were spiked with high concentrations of Human Collagen II and diluted with Reference Standard & Sample Diluent to produce samples with values ...read more
ELISA: Human Collagen II ELISA Kit (Chemiluminescence) [NBP2-75852] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
125.00-8000 pg/mL
Sensitivity
75.00 pg/mL

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Human Collagen II ELISA Kit (Chemiluminescence) Summary

Specificity
This kit recognizes Human COL2a1 in samples. No significant cross-reactivity or interference between Human COL2a1 and analogues was observed.
Standard Curve Range
125.00-8000 pg/mL
Sensitivity
75.00 pg/mL
Assay Type
Sandwich-CLIA
Inter-Assay
CV% < 11.06%
Intra-Assay
CV% < 10.91%
Spike Recovery
84-113%
Sample Volume
100 uL
Kit Type
ELISA Kit (Chemiluminescence)
Gene
COL2A1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro CLIA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Substrate Reagent A
  12. Substrate Reagent B

Alternate Names for Human Collagen II ELISA Kit (Chemiluminescence)

  • Alpha-1 type II collagen
  • ANFH
  • AOM
  • cartilage collagen
  • Chondrocalcin
  • COL11A3
  • COL2A1
  • Collagen 2
  • collagen alpha-1(II) chain
  • Collagen II
  • collagen II, alpha-1 polypeptide
  • collagen type 2
  • collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphysealdysplasia, congenital)
  • collagen, type II, alpha 1
  • Collagen-2
  • MGC131516
  • SEDC

Background

The extensive family of COL gene products (collagens) is composed of several chain types, including fibril-forming interstitial collagens (types I, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue, such as tendons and cartilage. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Several collagens also play a role in cell adhesion, important for maintaining normal tissue architecture and function. In cartilage, Collagen Type II constitutes the bulk of the fibril. Sensitization with Collagen Type II induces an erosive polyarthritis in rats, mice and higher primates which can resemble rheumatoid arthritis and relapsing polychrondritis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol COL2A1