![Western Blot: CNOT3 Antibody (4B8) [H00004849-M01] - Analysis of CNOT3 expression in transfected 293T cell line by CNOT3 monoclonal antibody (M01), clone 4B8.Lane 1: CNOT3 transfected lysate(82 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CNOT3-Antibody-4B8-Western-Blot-H00004849-M01-img0009.jpg "Western Blot: CNOT3 Antibody (4B8) [H00004849-M01] - Analysis of CNOT3 expression in transfected 293T cell line by CNOT3 monoclonal antibody (M01), clone 4B8.Lane 1: CNOT3 transfected lysate(82 KDa).Lane 2: Non-transfected lysate.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IP |
| Clone | 4B8 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | CNOT3 (NP_055331, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MADKRKLQGEIDRCLKKVSEGVEQFEDIWQKLHNAANANQKEKYEADLKKEIKKLQRLRDQIKTWVASNEIKDKRQLIDNRKLIETQMERFKVVERETKT |
| Localization | Cytoplasmic and Nuclear |
| Specificity | CNOT3 - CCR4-NOT transcription complex, subunit 3 |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CNOT3 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Application Notes | Antibody reactivity against cell lysate, tissue lysate and recombinant protein for WB. It has been used for IF and ELISA. Knock Out Validation was reported in scientific literature (PMID: 27746116). |
|
| Publications |
|
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: CNOT3 Antibody (4B8) [H00004849-M01] - Analysis of monoclonal antibody to CNOT3 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/CNOT3-Antibody-4B8-Immunocytochemistry-Immunofluorescence-H00004849-M01-img0006.jpg "Immunocytochemistry/Immunofluorescence: CNOT3 Antibody (4B8) [H00004849-M01] - Analysis of monoclonal antibody to CNOT3 on HeLa cell. Antibody concentration 10 ug/ml.")
![Western Blot: CNOT3 Antibody (4B8) [H00004849-M01] - CNOT3 monoclonal antibody (M01), clone 4B8 Analysis of CNOT3 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/CNOT3-Antibody-4B8-Western-Blot-H00004849-M01-img0008.jpg "Western Blot: CNOT3 Antibody (4B8) [H00004849-M01] - CNOT3 monoclonal antibody (M01), clone 4B8 Analysis of CNOT3 expression in Hela S3 NE.")
![ELISA: CNOT3 Antibody (4B8) [H00004849-M01] - Detection limit for recombinant GST tagged CNOT3 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CNOT3-Antibody-4B8-ELISA-H00004849-M01-img0010.jpg "ELISA: CNOT3 Antibody (4B8) [H00004849-M01] - Detection limit for recombinant GST tagged CNOT3 is 0.3 ng/ml as a capture antibody.")
![Flow Cytometry CCR4 Antibody (205410) [Unconjugated]](https://images.novusbio.com/images2/CCR4_MAB1567_Flow_Cytometry_20627.jpg)
![Immunohistochemistry CD55/DAF Antibody [Unconjugated]](https://images.novusbio.com/images2/CD55_AF2009_Immunohistochemistry_6728.jpg)
![Immunocytochemistry CD55/DAF Antibody [Unconjugated]](https://images.novusbio.com/images2/CD55_AF2009_Immunocytochemistry__Immunofluorescence_23052.jpg)
![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")