Novus Biologicals products are now on bio-techne.com

CLEC10A/CD301 Antibody (744812) [Alexa Fluor® 488]

Images

 
Human immature dendritic cells were stained with Mouse Anti-Human CLEC10A/CD301 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB48881G, filled histogram) or isotype control antibody (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
744812
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 488

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

CLEC10A/CD301 Antibody (744812) [Alexa Fluor® 488] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human CLEC10A/CD301
Gln61-His316
Accession # Q8IUN9
Specificity
Detects human CLEC10A/CD301 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human ASGPR1 or recombinant mouse MGL1 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CLEC10A
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 5 uL/10^6 cells

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CLEC10A/CD301 Antibody (744812) [Alexa Fluor® 488]

  • CD301 antigen
  • CD301
  • CD301C-type lectin domain family 10 member A
  • CLEC10A
  • CLECSF13
  • CLECSF14
  • CLECSF14macrophage C-type lectin
  • C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 14 (macrophage-derived)
  • C-type lectin domain family 10, member A
  • C-type lectin superfamily member 14
  • HML2
  • HMLC-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 13 (macrophage-derived)
  • macrophage lectin 2 (calcium dependent)
  • Macrophage lectin 2
  • MGL

Background

CLEC10A, also known as macrophage galactose/N-acetyl-galactosamine (GalNAc) specific lectin (MGL), CD301, DC-ASGPR, and HML, is a 40 kDa type II transmembrane glycoprotein that belongs to the C-type lectin family (1). Human and rat carry a single gene for CLEC10A/CD301, while mouse has two closely related MGL1 and MGL2 genes. Human CLEC10A/CD301 consists of a 39 amino acid (aa) cytoplasmic region, a 21 aa transmembrane segment and a 256 aa extracellular domain (ECD) with one carbohydrate recognition domain (CRD) and a neck region (2). Within the CRD, human CLEC10A/CD301 shares 64%-70% aa sequence identity with mouse MGL1, mouse MGL2, and rat MGL. Alternate splicing generates multiple isoforms of human CLEC10A/CD301 with 27 aa, 3 aa, and/or 4 aa deletions within the ECD (3, 4). CLEC10A/CD301 is expressed on immature myleloid dendritic cells and alternatively activated (tolerogenic) macrophages and is upregulated by the immunosuppressant dexamethasone (3-7). CLEC10A/CD301 selectively binds and internalizes terminal nonsialylated alpha - or beta -linked GalNAc moieties on O-linked carbohydrates, including the Tn carcinoma antigen (2-4, 8, 9). Similar ligand preference is exhibited by mouse MGL2 but not MGL1 (10). CLEC10A/CD301 expressed on tolerogenic dendritic cells binds carbohydrate determinants on CD45 (RA, RB, and RC but not RO isoforms) expressed by T, NK, and B cells (6). This interaction inhibits effector T cell activation and induces their apoptosis (6). CLEC10A/CD301 also binds the GP envelope glycoprotein on Marburg and Ebola viruses and enhances viral entry and infectivity (11).

  1. Zelensky, A.N. and J.E. Gready (2005) FEBS J. 272:6179.
  2. Suzuki, N. et al. (1996) J. Immunol. 156:128. 
  3. Higashi, N. et al. (2002) J. Biol. Chem. 277:20686. 
  4. Valladeau, J. et al. (2001) J. Immunol. 167:5767.
  5. van Vliet, S.J. et al. (2006) Immunobiology 211:577. 
  6. van Vliet, S.J. et al. (2006) Nat. Immunol. 7:1200.
  7. Raes, G. et al. (2005) J. Leukoc. Biol. 77:321.
  8. van Vliet, S.J. et al. (2005) Int. Immunol. 17:661.
  9. Higashi, N. et al. (2002) Int. Immunol. 14:545.
  10. Tsuiji, M. et al. (2002) J. Biol. Chem. 277:28892.
  11. Takada, A. et al. (2004) J. Virol. 78:2943.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-56745
Species: Hu, Mu
Applications: ChIP, ChIP, ICC/IF, IHC, IHC-P, IP, WB
NBP2-00735
Species: Hu, Pm
Applications: Flow, ICC/IF, IHC, IHC-P, WB
H00002166-M07
Species: Hu, Mu, Rt
Applications: ELISA, WB
NB100-1923
Species: Ce
Applications: IHC, IHC-P, IP, WB
NBP2-45564
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NLS32
Species: Bt, Bv, Ca, Eq, Hu, Pm, Mu, Po, Pm, Rt
Applications: ICC/IF, IHC, IHC-P
NBP1-97507
Species: Bv, Ca, Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, Simple Western, WB
NB100-292
Species: Hu
Applications: ICC/IF, IHC, IHC-P, IP, WB
NBP1-49938
Species: Hu, Mu
Applications: IHC, IHC-P, IP, WB
AF3767
Species: Hu, Mu, Rt
Applications: IHC, WB
AF4479
Species: Hu
Applications: IHC
NBP2-07996
Species: Hu
Applications: WB
NBP2-52486
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, WB
MAB1417
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
AF7197
Species: Hu, Mu
Applications: ICC, IHC, WB
H00008294-P01
Species: Hu
Applications: ELISA, AP, PA, PAGE, WB
NBL1-11570
Species: Hu
Applications: WB
FAB48881G
Species: Hu
Applications: Flow

Publications for CLEC10A/CD301 Antibody (FAB48881G) (0)

There are no publications for CLEC10A/CD301 Antibody (FAB48881G).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for CLEC10A/CD301 Antibody (FAB48881G) (0)

There are no reviews for CLEC10A/CD301 Antibody (FAB48881G). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CLEC10A/CD301 Antibody (FAB48881G) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CLEC10A/CD301 Antibody (744812) [Alexa Fluor® 488] and receive a gift card or discount.

Bioinformatics

Gene Symbol CLEC10A
Uniprot