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CLC Antibody

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Western Blot: CLC Antibody [NBP1-89926] - Analysis in control (vector only transfected HEK293T lysate) and CLCF1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: CLC Antibody [NBP1-89926] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: CLC Antibody [NBP1-89926] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: CLC Antibody [NBP1-89926] - Staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: CLC Antibody [NBP1-89926] - Staining of human fallopian tube shows moderate positivity in plasma in blood vessels.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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CLC Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: KTYDLTRYLEHQLRSLAGTYLNYLGPPFNEPDFNPPRLGAETLPRATVDLEVWRSLNDKLRLTQNYE
Predicted Species
Mouse (97%), Rat (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CLCF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
CLC Recombinant Protein Antigen (NBP1-89926PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CLC Antibody

  • B-cell stimulating factor 3
  • B-cell stimulatory factor 3
  • BSF-3
  • BSF3BSF-3
  • cardiotrophin-like cytokine factor 1
  • CISS2
  • CLC
  • CLCF1
  • CLCNNT-1
  • CRLF1 associated cytokine-like factor 1
  • neurotrophin-1/B-cell stimulating factor-3
  • NNT-1
  • NNT1B-cell-stimulating factor 3
  • Novel neurotrophin-1
  • NR6

Background

The CLCF1 gene is a member of the glycoprotein (gp)130 cytokine family and encodes cardiotrophin-like cytokine factor 1(CLCF1). CLCF1 forms a heterodimer complex with cytokine receptor-like factor 1 (CRLF1). This dimer competes with ciliary neurotrophic factor

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CLCF1