![Immunocytochemistry/Immunofluorescence: Claudin-3 Antibody (HL1527) - Azide and BSA Free [NBP3-25412] - Claudin 3 antibody [HL1527] detects Claudin 3 protein at cell membrane by immunofluorescent analysis. Sample: MCF-7 and HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Claudin 3 stained by Claudin 3 antibody [HL1527] (NBP3-25412) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25412_rabbit-claudin-3-mab-hl1527-azide-and-bsa-free-212024939358.jpg "Immunocytochemistry/Immunofluorescence: Claudin-3 Antibody (HL1527) - Azide and BSA Free [NBP3-25412] - Claudin 3 antibody [HL1527] detects Claudin 3 protein at cell membrane by immunofluorescent analysis. Sample: MCF-7 and HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Claudin 3 stained by Claudin 3 antibody [HL1527] (NBP3-25412) diluted at 1:500. Blue: Fluoroshield with DAPI .")
| Reactivity | Hu, RMSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | HL1527 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Claudin-3. The exact sequence is proprietary. |
| Predicted Species | Rhesus Macaque (90%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | CLDN3 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Claudin-3 Antibody (NBP3-25412)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | CLDN3 |
![Immunohistochemistry-Paraffin: Claudin-3 Antibody (HL1527) - Azide and BSA Free [NBP3-25412] - Claudin 3 antibody [HL1527] detects Claudin 3 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human colon. Claudin 3 stained by Claudin 3 antibody [HL1527] (NBP3-25412) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25412_rabbit-claudin-3-mab-hl1527-azide-and-bsa-free-212024943552.jpg "Immunohistochemistry-Paraffin: Claudin-3 Antibody (HL1527) - Azide and BSA Free [NBP3-25412] - Claudin 3 antibody [HL1527] detects Claudin 3 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human colon. Claudin 3 stained by Claudin 3 antibody [HL1527] (NBP3-25412) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: Claudin-3 Antibody (HL1527) - Azide and BSA Free [NBP3-25412] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Claudin 3 antibody [HL1527] (NBP3-25412) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25412_rabbit-claudin-3-mab-hl1527-azide-and-bsa-free-212024940896.jpg "Western Blot: Claudin-3 Antibody (HL1527) - Azide and BSA Free [NBP3-25412] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Claudin 3 antibody [HL1527] (NBP3-25412) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")