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Human Claudin-18 ELISA Kit (Colorimetric)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Tissue Homogenates, Cell Lysates And Other Biological Fluids
Standard Curve Range
0.156 - 10 ng/mL
Sensitivity
0.055 ng/mL

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Human Claudin-18 ELISA Kit (Colorimetric) Summary

Description
Assay time: 3 hours
Standard Curve Range
0.156 - 10 ng/mL
Sensitivity
0.055 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 12
Intra-Assay
%CV < 10
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CLDN18

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Assay Diluent A
  2. Assay Diluent B
  3. Detection Reagent A
  4. Detection Reagent B
  5. Instruction manual
  6. Plate sealer for 96 wells
  7. Pre-coated ready to use 96-well strip plate
  8. Standard Diluent
  9. Standard
  10. Stop Solution
  11. TMB Substrate
  12. Wash Buffer (30 x concentrate)

Alternate Names for Human Claudin-18 ELISA Kit (Colorimetric)

  • claudin 18
  • Claudin18
  • Claudin-18
  • CLDN18
  • SFTA5
  • SFTPJ
  • surfactant associated 5
  • surfactant associated protein J
  • surfactant, pulmonary associated protein J

Background

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is upregulated in patients with ulcerative colitis and highly overexpressed in infiltrating ductal adenocarcinomas. PKC/MAPK/AP-1 (protein kinase C/mitogen-activated protein kinase/activator protein-1) dependent pathway regulates the expression of this gene in gastric cells. Alternatively spliced transcript variants encoding different isoforms have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol CLDN18