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Chromogranin A Antibody (OTI2E12) [Janelia Fluor® 646]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, ICC/IF
Clone
OTI2E12
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 646

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-70410AF350
Alexa Fluor 405
NBP2-70410AF405
Alexa Fluor 488
NBP2-70410AF488
Alexa Fluor 532
NBP2-70410AF532
Alexa Fluor 594
NBP2-70410AF594
Alexa Fluor 647
NBP2-70410AF647
Alexa Fluor 700
NBP2-70410AF700
Alexa Fluor 750
NBP2-70410AF750
Allophycocyanin
NBP2-70410APC
Biotin
NBP2-70410B
CoraFluor 1
NBP2-70410CL1
DyLight 350
NBP2-70410UV
DyLight 405
NBP2-70410V
DyLight 488
NBP2-70410G
DyLight 550
NBP2-70410R
DyLight 594
NBP2-70410DL594
DyLight 650
NBP2-70410C
DyLight 680
NBP2-70410FR
DyLight 755
NBP2-70410IR
FITC
NBP2-70410F
HRP
NBP2-70410H
Janelia Fluor 525
NBP2-70410JF525
Janelia Fluor 549
NBP2-70410JF549
Janelia Fluor 669
NBP2-70410JF669
PE
NBP2-70410PE
PerCP
NBP2-70410PCP
Unconjugated
NBP2-46282
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-46282
Azide and BSA Free
NBP2-70410
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Chromogranin A Antibody (OTI2E12) [Janelia Fluor® 646] Summary

Immunogen
Full length recombinant protein of human Chromogranin A (NP_001266) produced in HEK293T cells.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CHGA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunofluorescence
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Chromogranin A Antibody (OTI2E12) [Janelia Fluor® 646]

  • betagranin (N-terminal fragment of chromogranin A)
  • Betagranin
  • Catestatin
  • CGA
  • CHGA
  • Chromofungin
  • chromogranin A (parathyroid secretory protein 1)
  • Chromogranin A
  • chromogranin-A
  • Pancreastatin
  • Parastatin
  • Parathyroid Secretory Protein 1
  • Pituitary Secretory Protein I
  • SP-I

Background

Chromogranin A (CgA) is an 86 kDa protein that is the major member of the granin family of acidic secretory glycoproteins located in neuroendocrine cells. CgA is believed to play a role in targeting peptide hormones and neurotransmitters to granules of the regulated pathways and inhibit hormone and neurotransmitter release. Also, the widespread distribution of CgA has made the measurement of circulating immunoreactive CgA a valuable tool in the diagnosis of neuroendocrine neoplasia, and CgA immunohistochemistry can help to identify the neuroendocrine nature of tumors. The N-terminal domain of CgA inhibits tumor necrosis factor a (TNFa) induced gap formation in human umbilical venous endothelial cells. CgA levels which reflect neuroendocrine differentiation of prostatic carcinoma may have a diagnostic, therapeutic and prognostic role in the management of prostate cancer patients.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Chromogranin A Antibody (NBP2-70410JF646). (Showing 1 - 1 of 1 FAQ).

  1. I have following question about antibodies against Chromogranin A. Your products list the species but canine is not included. Does it mean that the products cannot be used on canine tissues?
    • When we do not list a species, it means we have not tested an antibody to work with those samples. If we test an antibody and find that it does not work, we will list the species with a minus sign. Unfortunately, we haven't tried these abs on canine tissues and they may work, but we cannot guarantee it. If you would like to purchase one to try, you will be eligible for the Innovators Reward Program.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CHGA