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Chromogranin A Antibody (CGA/414)

Images

 
Immunohistochemistry-Paraffin: Chromogranin A Antibody (CGA/414) [NBP2-29428] - Formalin-fixed, paraffin-embedded pancreas stained with Chromogranin A Ab (CGA414).
Immunohistochemistry-Paraffin: Chromogranin A Antibody (CGA/414) [NBP2-29428] - Formalin-fixed, paraffin-embedded human small cell Lung Carcinoma stained with Chromogranin A Monoclonal Antibody (CGA/414)

Product Details

Summary
Reactivity Hu, Mu, Rt(-)Species Glossary
Applications ICC/IF, IHC
Clone
CGA/414
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

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Chromogranin A Antibody (CGA/414) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33198)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
Recombinant full-length human Chromogranin A protein (Uniprot: P10645)
Localization
Finely granular cytoplasmic
Marker
Neuroendocrine Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CHGA
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1-2ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Publications
Read Publications using
NBP2-29428 in the following applications:

Reactivity Notes

Does not react with Rat.

Mouse reactivity reported in scientific literature (PMID: 29352046).

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for Chromogranin A Antibody (CGA/414)

  • betagranin (N-terminal fragment of chromogranin A)
  • Betagranin
  • Catestatin
  • CGA
  • CHGA
  • Chromofungin
  • chromogranin A (parathyroid secretory protein 1)
  • Chromogranin A
  • chromogranin-A
  • Pancreastatin
  • Parastatin
  • Parathyroid Secretory Protein 1
  • Pituitary Secretory Protein I
  • SP-I

Background

Chromogranin A is found in secretory vesicles of neurons and endocrine cells. It is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Chromogranin A Antibody (NBP2-29428)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: ELISA, ICC/IF, IF/IHC, IHC-P, Immunocytochemistry/ Immunofluorescence, Western Blot.


Filter By Application
ELISA
(1)
ICC/IF
(1)
IF/IHC
(1)
IHC-P
(1)
Immunocytochemistry/ Immunofluorescence
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using NBP2-29428 Applications Species
Zhou, J;Sheridan, MA;Tian, Y;Dahlgren, KJ;Messler, M;Peng, T;Ezashi, T;Schulz, LC;Ulery, BD;Roberts, RM;Schust, DJ; Development of properly-polarized trophoblast stem cell-derived organoids to model early human pregnancy. bioRxiv : the preprint server for biology 2023-10-02 [PMID: 37873440]
Che YH, Choi IY, Song CE et al. Peripheral Neuron-Organoid Interaction Induces Colonic Epithelial Differentiation via Non-Synaptic Substance P Secretion International Journal of Stem Cells 2023-08-30 [PMID: 37385635] (ELISA, Western Blot, Immunocytochemistry/ Immunofluorescence) ELISA, Western Blot, Immunocytochemistry/ Immunofluorescence
Sampedro-NUNez M, Bouthelier A, Serrano-Somavilla A et al LAT-1 and GLUT-1 Carrier Expression and Its Prognostic Value in Gastroenteropancreatic Neuroendocrine Tumors Cancers (Basel) 2020-10-17 [PMID: 33066332] (IHC-P, Human)

Details:
Citation using the Azide and BSA Free version of this antibody.
IHC-P Human
Workman MJ, Gleeson JP, Troisi EJ et al. Enhanced Utilization of Induced Pluripotent Stem Cell-Derived Human Intestinal Organoids Using Microengineered Chips Cell Mol Gastroenterol Hepatol 2017-12-29 [PMID: 29930984] (ICC/IF, Human) ICC/IF Human
Jiang Z, Rajamanickam S, Justice NJ. Local Corticotropin-Releasing-Factor Signaling in the Hypothalamic Paraventricular Nucleus J. Neurosci. 2018-01-19 [PMID: 29352046] (Mouse) Mouse
Sampedro-Nunez M, Luque RM, Ramos-Levi AM et al. Presence of sst5TMD4, a truncated splice variant of the somatostatin receptor subtype 5, is associated to features of increased aggressiveness in pancreatic neuroendocrine tumors. Oncotarget [PMID: 26673010] (IF/IHC)

Details:
Used the Alexa Fluor 488 form of this antibody.
IF/IHC
Zhou J, Sheridan M, Tian Y et al. Development of Properly-Polarized Trophoblast Stem Cell-Derived Organoids to Model Early Human Pregnancy SSRN Electronic Journal 2022-11-02

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for Chromogranin A Antibody (NBP2-29428). (Showing 1 - 1 of 1 FAQs).

  1. I have following question about antibodies against Chromogranin A. Your products list the species but canine is not included. Does it mean that the products cannot be used on canine tissues?
    • When we do not list a species, it means we have not tested an antibody to work with those samples. If we test an antibody and find that it does not work, we will list the species with a minus sign. Unfortunately, we haven't tried these abs on canine tissues and they may work, but we cannot guarantee it. If you would like to purchase one to try, you will be eligible for the Innovators Reward Program.

Secondary Antibodies

 

Isotype Controls

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Research Areas for Chromogranin A Antibody (NBP2-29428)

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Blogs on Chromogranin A.

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Bioinformatics

Gene Symbol CHGA
Uniprot