![Immunohistochemistry-Paraffin: Chromogranin A Antibody (CGA/413 + CHGA/777 + CHGA/798) [NBP2-44764] - Pheochromocytoma stained with Chromogranin A Monoclonal Antibody (CGA/413+ CHGA/777+ CHGA/798)](http://images.novusbio.com/fullsize/Chromogranin-A-Antibody-CGA-413-+-CHGA-777-+-CHGA-798-Immunohistochemistry-Paraffin-NBP2-44764-img0004.jpg "Immunohistochemistry-Paraffin: Chromogranin A Antibody (CGA/413 + CHGA/777 + CHGA/798) [NBP2-44764] - Pheochromocytoma stained with Chromogranin A Monoclonal Antibody (CGA/413+ CHGA/777+ CHGA/798)")
| Reactivity | Hu, Mu, Rt, Po, PmSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | CGA/413 + CHGA/777 + CHGA/798 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47846) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Recombinant human Chromogranin A protein (Uniprot: P10645) |
| Localization | Finely granular cytoplasmic |
| Marker | Neuroendocrine Marker |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CHGA |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. Use in ICC/IF, WB reported in scientific literature (PMID:34381974). |
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| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
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| Publication using NBP2-44764 | Applications | Species |
|---|---|---|
| Xiang C, Chen P, Zhang Q et al. Intestinal microbiota modulates adrenomedullary response through Nod1 sensing in chromaffin cells iScience 2021-08-01 [PMID: 34381974] (ICC/IF, WB, IHC-P) | ICC/IF, WB, IHC-P |
Secondary Antibodies |
Isotype Controls |
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Beta Tubulin III and neurogenesis Beta tubulin III, also known as Tuj-1, is a class III member of the beta tubulin protein family. Beta tubulins are one of two structural components that form our microtubule network. While general tubulins play a role in a wide range of cellular pr... Read full blog post. |
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![Immunohistochemistry-Paraffin: Chromogranin A Antibody (CGA/413 + CHGA/777 + CHGA/798) [NBP2-44764] - Adrenal Gland stained with Chromogranin A Monoclonal Antibody (CGA/413+ CHGA/777+ CHGA/798)](http://images.novusbio.com/fullsize/Chromogranin-A-Antibody-CGA-413-+-CHGA-777-+-CHGA-798-Immunohistochemistry-Paraffin-NBP2-44764-img0001.jpg "Immunohistochemistry-Paraffin: Chromogranin A Antibody (CGA/413 + CHGA/777 + CHGA/798) [NBP2-44764] - Adrenal Gland stained with Chromogranin A Monoclonal Antibody (CGA/413+ CHGA/777+ CHGA/798)")
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