![Immunocytochemistry/Immunofluorescence: Choline Acetyltransferase/ChAT Antibody (JA67-11) [NBP2-66779] - Staining Choline Acetyltransferase in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-JA67-11-Immunocytochemistry-Immunofluorescence-NBP2-66779-img0004.jpg "Immunocytochemistry/Immunofluorescence: Choline Acetyltransferase/ChAT Antibody (JA67-11) [NBP2-66779] - Staining Choline Acetyltransferase in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IP |
| Clone | JA67-11 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within Human Choline Acetyltransferase/ChAT aa 699-748 / 748. (SwissProt: P28329 Human; SwissProt: Q03059 Mouse; SwissProt: P32738 Rat) |
| Localization | Cytosol, nucleus, cytoplasm, neuron projection, presynapse |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | CHAT |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Choline Acetyltransferase/ChAT Antibody (NBP2-66779)Find related products by research area.
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Choline Acetyltransferase (ChAT) – a useful Immunohistochemical marker for morphological studies of neurons Choline Acetyltransferase (ChAT) is the enzyme that is responsible for biosynthesis of the neurotransmitter acetylcholine. The majority of acetylcholine is synthesized locally at nerve terminals where ChAT catalyzes the transfer of an acetyl group ... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | CHAT |
![Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (JA67-11) [NBP2-66779] - Analysis of paraffin-embedded rat stomach tissue using anti- Choline Acetyltransferase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-JA67-11-Immunohistochemistry-Paraffin-NBP2-66779-img0006.jpg "Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (JA67-11) [NBP2-66779] - Analysis of paraffin-embedded rat stomach tissue using anti- Choline Acetyltransferase antibody. Counter stained with hematoxylin.")
![Flow Cytometry: Choline Acetyltransferase/ChAT Antibody (JA67-11) [NBP2-66779] - Analysis of N2A cells with Choline Acetyltransferase antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-JA67-11-Flow-Cytometry-NBP2-66779-img0005.jpg "Flow Cytometry: Choline Acetyltransferase/ChAT Antibody (JA67-11) [NBP2-66779] - Analysis of N2A cells with Choline Acetyltransferase antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.")
![Immunocytochemistry/Immunofluorescence: Choline Acetyltransferase/ChAT Antibody (JA67-11) [NBP2-66779] - Staining ChoLine Acetyltransferase in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-JA67-11-Immunocytochemistry-Immunofluorescence-NBP2-66779-img0001.jpg "Immunocytochemistry/Immunofluorescence: Choline Acetyltransferase/ChAT Antibody (JA67-11) [NBP2-66779] - Staining ChoLine Acetyltransferase in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunocytochemistry/Immunofluorescence: Choline Acetyltransferase/ChAT Antibody (JA67-11) [NBP2-66779] - Staining Choline Acetyltransferase in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-JA67-11-Immunocytochemistry-Immunofluorescence-NBP2-66779-img0003.jpg "Immunocytochemistry/Immunofluorescence: Choline Acetyltransferase/ChAT Antibody (JA67-11) [NBP2-66779] - Staining Choline Acetyltransferase in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (JA67-11) [NBP2-66779] - Analysis of paraffin-embedded mouse brain tissue using anti- Choline Acetyltransferase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-JA67-11-Immunohistochemistry-Paraffin-NBP2-66779-img0002.jpg "Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (JA67-11) [NBP2-66779] - Analysis of paraffin-embedded mouse brain tissue using anti- Choline Acetyltransferase antibody. Counter stained with hematoxylin.")
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![Western Blot GAD1/GAD67 Antibody [Unconjugated]](https://images.novusbio.com/images2/GAD1_AF2086_Western_Blot_19950.jpg)
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")