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Porcine Cholecystokinin ELISA Kit (Colorimetric)

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Product Details

Summary
Reactivity PoSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
Standard Curve Range
12.35 - 1000 pg/mL
Sensitivity
4.45 pg/mL

Order Details

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Porcine Cholecystokinin ELISA Kit (Colorimetric) Summary

Description
Assay time: 2 hours
Standard Curve Range
12.35 - 1000 pg/mL
Sensitivity
4.45 pg/mL
Assay Type
Competitive ELISA
Inter-Assay
%CV < 12
Intra-Assay
%CV < 10
Spike Recovery
89 - 103%
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CCK

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Assay Diluent A
  2. Assay Diluent B
  3. Detection Reagent A
  4. Detection Reagent B
  5. Instruction manual
  6. Plate sealer for 96 wells
  7. Pre-coated ready to use 96-well strip plate
  8. Standard Diluent
  9. Standard
  10. Stop Solution
  11. TMB Substrate
  12. Wash Buffer (30 x concentrate)

Alternate Names for Porcine Cholecystokinin ELISA Kit (Colorimetric)

  • CCK
  • cholecystokinin
  • MGC117187

Background

The regulated translation of messenger RNA is essential for cell-cycle progression, establishment of the body plan during early development and modulation of key activities in the central nervous system. Cytoplasmic polyadenylation, one mechanism of controlling translation, is driven by cytoplasmic polyadenylation element binding protein, CPEB. CPEB is a highly conserved, sequence-specific RNA-binding protein that binds to the cytoplasmic polyadenylation element, thereby modulating translational repression and mRNA localization. Blocking cytoplasmic polyadenylation by interfering with the CPE or CPEB prevents the translational activation and translational repression of mRNAs crucial for oocyte maturation. CPEB is synthesized during oogenesis and stockpiled in the oocyte. CPEB degradation occurs via the proteasome pathway, most likely through ubiquitin-conjugated intermediates.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol CCK