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Cholecystokinin Antibody (2753A) [Unconjugated]

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CCK was detected in immersion fixed Capan‑2 human pancreatic adenocarcinoma cell line (positive staining) and Daudi human Burkitt's lymphoma cell line (negative staining) using Rabbit Anti-Human CCK Monoclonal Antibody (Catalog # MAB11040) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
CCK was detected in immersion fixed Capan‑2 human pancreatic adenocarcinoma cell line (positive staining) and Daudi human Burkitt's lymphoma cell line (negative staining) using Rabbit Anti-Human CCK Monoclonal ...read more
CCK was detected in immersion fixed paraffin-embedded sections of human duodenum using Rabbit Anti-Human CCK Monoclonal Antibody (Catalog # MAB11040) at 3 µg/mL for 1 hour at room temperature followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, ICC/IF
Clone
2753A
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB11040-SP
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Cholecystokinin Antibody (2753A) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Human CCK synthetic peptide
Accession # P06307
Specificity
Detects human CCK in direct ELISAs.
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Purity Statement
Protein A or G purified from cell culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 3-25 ug/mL
  • Immunohistochemistry 3-25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cholecystokinin Antibody (2753A) [Unconjugated]

  • CCK
  • cholecystokinin
  • MGC117187

Background

Cholecystokinin (CCK) is a peptide hormone secreted by the gastrointestinal system that stimulates the digestion of protein and fat.  CCK is synthesized and secreted by enteroendocrine cells of the duodenum and acts as a hunger suppressant. CCK participates in several processes such as digestion, satiety, and anxiety. CCK is also found extensively throughout the central nervous system with anxiogenic, panicogenic and hallucinogenic effects.  Receptors of CCK are found in the central nervous system specifically in the hippocampus, cerebral cortex, and striatum.

Lenka A., Arumugham SS., Christopher R., Pal PK, "Genetic Substrates of Psychosis in Patients with Parkinson's Disease: A Critical Review". Journal of the Neurological Sciences. 2016 May; 364: 33-41. Zwanzger P., Domschke K., Bradwejn J., "Neuronal Network of Panic Disorder: The Role of the Neuropeptide Cholecystokinin". Depression and Anxiety. 2012 Sep. 29(9): 762-74. Okonkwo O., Zezoff D., Adeyinka A., "Biochemistry, Cholecystokinin". StatPearls. 2021 Jan. Available from: https://www.ncbi.nlm.nih.gov/books/NBK534204/.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Cholecystokinin
Antibody (2753A) [Unc ...
MAB11040
Cholecystokinin Antibody (2753A) [Unconjugated]
Species: Hu
Applications: IHC, ICC/IF

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