![Western Blot: CHMP5 Antibody [NBP1-32597] - Jurkat whole cell extracts and Jurkat exosome extract (15.4 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CHMP5 antibody [N1C3] diluted at 1:250. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CHMP5-Antibody-Western-Blot-NBP1-32597-img0009.jpg "Western Blot: CHMP5 Antibody [NBP1-32597] - Jurkat whole cell extracts and Jurkat exosome extract (15.4 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CHMP5 antibody [N1C3] diluted at 1:250. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, Po, Bv, Ch, RMSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CHMP5. The exact sequence is proprietary. |
| Localization | Cytoplasm, cytosol, Endosome membrane, Peripheral membrane protein, Cytoplasm, cytosol, Endosome membrane |
| Predicted Species | Rat (98%), Porcine (100%), Bovine (100%), Rhesus Macaque (100%), Chicken (94%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CHMP5 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 25 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS, 20% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for CHMP5 Antibody (NBP1-32597)Find related products by research area.
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| Gene Symbol | CHMP5 |
![Immunocytochemistry/Immunofluorescence: CHMP5 Antibody [NBP1-32597] - HL-60 cells were fixed in ice-cold MeOH for 5 min. Green: CHMP5 protein stained by CHMP5 antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/CHMP5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32597-img0006.jpg "Immunocytochemistry/Immunofluorescence: CHMP5 Antibody [NBP1-32597] - HL-60 cells were fixed in ice-cold MeOH for 5 min. Green: CHMP5 protein stained by CHMP5 antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: CHMP5 Antibody [NBP1-32597] - Mouse urinary bladder. CHMP5 stained by CHMP5 antibody [N1C3] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/CHMP5-Antibody-Immunohistochemistry-Paraffin-NBP1-32597-img0007.jpg "Immunohistochemistry-Paraffin: CHMP5 Antibody [NBP1-32597] - Mouse urinary bladder. CHMP5 stained by CHMP5 antibody [N1C3] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: CHMP5 Antibody [NBP1-32597] - Sample(30ug whole cell lysate)A: 293TB: A431 C: H1299D: HeLa S3 E: Hep G2 2% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/CHMP5-Antibody-Western-Blot-NBP1-32597-img0005.jpg "Western Blot: CHMP5 Antibody [NBP1-32597] - Sample(30ug whole cell lysate)A: 293TB: A431 C: H1299D: HeLa S3 E: Hep G2 2% SDS PAGE, antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: CHMP5 Antibody [NBP1-32597] - NCIN87 xenograft, using CHMP5 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/CHMP5-Antibody-Immunohistochemistry-Paraffin-NBP1-32597-img0003.jpg "Immunohistochemistry-Paraffin: CHMP5 Antibody [NBP1-32597] - NCIN87 xenograft, using CHMP5 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: CHMP5 Antibody [NBP1-32597] -CHMP5 antibody detects CHMP5 protein by Western blot analysis. Various whole cell extracts (30 ug) were separated by 15 % SDS-PAGE, and blotted with CHMP5 antibody diluted by 1:1000](http://images.novusbio.com/fullsize/antibody/nbp1-32597_rabbit-polyclonal-chmp5-antibody-western-blot-3172024123837..jpg "Western Blot: CHMP5 Antibody [NBP1-32597] -CHMP5 antibody detects CHMP5 protein by Western blot analysis. Various whole cell extracts (30 ug) were separated by 15 % SDS-PAGE, and blotted with CHMP5 antibody diluted by 1:1000")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")