![Western Blot: CDX2 Antibody (SY09-02) [NBP2-66769] - Analysis of CDX2 on AGS cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Predicted band size: 34 kDa Observed band size: 40 kDa](http://images.novusbio.com/fullsize/CDX2-Antibody-SY09-02-Western-Blot-NBP2-66769-img0007.jpg "Western Blot: CDX2 Antibody (SY09-02) [NBP2-66769] - Analysis of CDX2 on AGS cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Predicted band size: 34 kDa Observed band size: 40 kDa")
| Reactivity | Hu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | SY09-02 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within human CDX2 aa 20-60. (SwissProt: Q99626 Human; Unigene: 64495 Rat) |
| Localization | Nucleus. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | CDX2 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
![Western Blot SOX2 Antibody [Unconjugated]](https://images.novusbio.com/images2/SOX2_AF2018_Western_Blot_5161.jpg)
![Chromatin Immunoprecipitation (ChIP) SOX2 Antibody [Unconjugated]](https://images.novusbio.com/images2/SOX2_AF2018_Chromatin_Immunoprecipitation_11224.jpg)
![Simple Western SOX2 Antibody [Unconjugated]](https://images.novusbio.com/images2/SOX2_AF2018_Simple_Western_16676.jpg)
![Western Blot beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/beta-Catenin_AF1329_Western_Blot_5100.jpg)
![Chromatin Immunoprecipitation (ChIP) beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/beta-Catenin_AF1329_Chromatin_Immunoprecipitation_11251.jpg)
![Simple Western beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/betaCatenin_AF1329_Simple_Western_17134.jpg)
![Immunohistochemistry EGFR Antibody [Unconjugated]](https://images.novusbio.com/images2/EGF_R_AF231_Immunohistochemistry_20892.jpg)
![Immunocytochemistry EGFR Antibody [Unconjugated]](https://images.novusbio.com/images2/EGF_R_AF231_Immunocytochemistry__Immunofluorescence_21143.jpg)
![Western Blot EGFR Antibody [Unconjugated]](https://images.novusbio.com/images2/EGF_R_AF231_Western_Blot_19925.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for CDX2 Antibody (NBP2-66769)Find related products by research area.
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Read full blog post. |
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Stemness is responsible for onset and metastasis of colorectal cancer By Jamshed Arslan, Pharm. D., PhD. Colorectal cancer stem cells are a rare subpopulation of colorectal cancer cells that can self-renew and initiate and sustain tumor growth when transplanted into an animal host.1,2 C... Read full blog post. |
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| Gene Symbol | CDX2 |
![Immunocytochemistry/Immunofluorescence: CDX2 Antibody (SY09-02) [NBP2-66769] - Staining CDX2 in LOVO cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CDX2-Antibody-SY09-02-Immunocytochemistry-Immunofluorescence-NBP2-66769-img0005.jpg "Immunocytochemistry/Immunofluorescence: CDX2 Antibody (SY09-02) [NBP2-66769] - Staining CDX2 in LOVO cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: CDX2 Antibody (SY09-02) [NBP2-66769] - Analysis of paraffin-embedded rat large intestine tissue with Rabbit anti-CDX2 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CDX2-Antibody-SY09-02-Immunohistochemistry-Paraffin-NBP2-66769-img0012.jpg "Immunohistochemistry-Paraffin: CDX2 Antibody (SY09-02) [NBP2-66769] - Analysis of paraffin-embedded rat large intestine tissue with Rabbit anti-CDX2 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Flow Cytometry: CDX2 Antibody (SY09-02) [NBP2-66769] - Analysis of Hela cells with CDX2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/CDX2-Antibody-SY09-02-Flow-Cytometry-NBP2-66769-img0001.jpg "Flow Cytometry: CDX2 Antibody (SY09-02) [NBP2-66769] - Analysis of Hela cells with CDX2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody")
![Western Blot: CDX2 Antibody (SY09-02) [NBP2-66769] - Analysis of CDX2 on AGS cells lysates using anti-CDX2 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/CDX2-Antibody-SY09-02-Western-Blot-NBP2-66769-img0006.jpg "Western Blot: CDX2 Antibody (SY09-02) [NBP2-66769] - Analysis of CDX2 on AGS cells lysates using anti-CDX2 antibody at 1/1,000 dilution.")
![Immunocytochemistry/Immunofluorescence: CDX2 Antibody (SY09-02) [NBP2-66769] - Staining CDX2 in AGS cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CDX2-Antibody-SY09-02-Immunocytochemistry-Immunofluorescence-NBP2-66769-img0004.jpg "Immunocytochemistry/Immunofluorescence: CDX2 Antibody (SY09-02) [NBP2-66769] - Staining CDX2 in AGS cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: CDX2 Antibody (SY09-02) [NBP2-66769] - Analysis of paraffin-embedded human colon cancer tissue using anti-CDX2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CDX2-Antibody-SY09-02-Immunohistochemistry-Paraffin-NBP2-66769-img0002.jpg "Immunohistochemistry-Paraffin: CDX2 Antibody (SY09-02) [NBP2-66769] - Analysis of paraffin-embedded human colon cancer tissue using anti-CDX2 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: CDX2 Antibody (SY09-02) [NBP2-66769] - Analysis of paraffin-embedded rat large intestine tissue using anti-CDX2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CDX2-Antibody-SY09-02-Immunohistochemistry-Paraffin-NBP2-66769-img0003.jpg "Immunohistochemistry-Paraffin: CDX2 Antibody (SY09-02) [NBP2-66769] - Analysis of paraffin-embedded rat large intestine tissue using anti-CDX2 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: CDX2 Antibody (SY09-02) [NBP2-66769] - Analysis of paraffin-embedded human colon tissue with Rabbit anti-CDX2 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CDX2-Antibody-SY09-02-Immunohistochemistry-Paraffin-NBP2-66769-img0008.jpg "Immunohistochemistry-Paraffin: CDX2 Antibody (SY09-02) [NBP2-66769] - Analysis of paraffin-embedded human colon tissue with Rabbit anti-CDX2 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry-Paraffin: CDX2 Antibody (SY09-02) [NBP2-66769] - Analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-CDX2 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CDX2-Antibody-SY09-02-Immunohistochemistry-Paraffin-NBP2-66769-img0009.jpg "Immunohistochemistry-Paraffin: CDX2 Antibody (SY09-02) [NBP2-66769] - Analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-CDX2 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry-Paraffin: CDX2 Antibody (SY09-02) [NBP2-66769] - Analysis of paraffin-embedded human appendix tissue with Rabbit anti-CDX2 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CDX2-Antibody-SY09-02-Immunohistochemistry-Paraffin-NBP2-66769-img0010.jpg "Immunohistochemistry-Paraffin: CDX2 Antibody (SY09-02) [NBP2-66769] - Analysis of paraffin-embedded human appendix tissue with Rabbit anti-CDX2 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry-Paraffin: CDX2 Antibody (SY09-02) [NBP2-66769] - Analysis of paraffin-embedded Human stomach cancer tissue with Rabbit anti-CDX2 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CDX2-Antibody-SY09-02-Immunohistochemistry-Paraffin-NBP2-66769-img0011.jpg "Immunohistochemistry-Paraffin: CDX2 Antibody (SY09-02) [NBP2-66769] - Analysis of paraffin-embedded Human stomach cancer tissue with Rabbit anti-CDX2 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Chromatin Immunoprecipitation (ChIP) Oct-3/4 Antibody [Unconjugated]](https://images.novusbio.com/images2/Oct-3_4_AF1759_Chromatin_Immunoprecipitation_11218.jpg)
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![Simple Western Oct-3/4 Antibody [Unconjugated]](https://images.novusbio.com/images2/Oct3_4_AF1759_Simple_Western_20183.jpg)
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")