CDC25C [p Ser216] Antibody

Images

 
Western Blot: CDC25C [p Ser216] Antibody [NB100-81841] - Analysis of extracts from 293 cells untreated or treated with serum using CDC25C [p Ser216] Antibody.
Immunocytochemistry/ Immunofluorescence: CDC25C [p Ser216] Antibody [NB100-81841] - Staining of methanol-fixed Hela cells using CDC25C [p Ser216] Antibody.
Immunohistochemistry-Paraffin: CDC25C [p Ser216] Antibody [NB100-81841] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NB100-81841 (left) or the same antibody preincubated with ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

CDC25C [p Ser216] Antibody Summary

Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human CDC25C around the phosphorylation site of serine 216 (S-P-SP-M-P).
Modification
p Ser216
Marker
Mitosis Marker
Specificity
Detects endogenous levels of CDC25C only when phosphorylated at serine 216.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CDC25C
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:200
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
CDC25C Overexpression Lysate

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CDC25C [p Ser216] Antibody

  • CDC 25
  • CDC25
  • CDC25C
  • cell division cycle 25 homolog C (S. cerevisiae)
  • cell division cycle 25 homolog C (S. pombe)
  • cell division cycle 25C
  • Dual specificity phosphatase Cdc25C
  • EC 3.1.3.48
  • mitosis inducer CDC25
  • M-phase inducer phosphatase 3
  • phosphotyrosine phosphatase

Background

Cdc25C is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It has been highly conserved during evolution and it plays a key role in the regulation of cell division. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Cdc25C is mainly expressed in G2 phase. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CDC25C Antibody (NB100-81841) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CDC25C
Entrez
Uniprot