CDC25C Antibodies
We offer Cdc25C Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Western Blot. Each Cdc25C Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Cdc25C Antibodies can be used in a variety of model species: Human. Use the list below to choose the Cdc25C Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Cdc25C polyclonal antibodies and browse our Cdc25C monoclonal antibody catalog.
Alternate Names for CDC25C Antibodies
anti-CDC25C antibody, anti-CDC 25 antibody, anti-CDC25 antibody, anti-cell division cycle 25 homolog C (S. cerevisiae) antibody, anti-cell division cycle 25 homolog C (S. pombe) antibody, anti-cell division cycle 25C antibody, anti-Dual specificity phosphatase Cdc25C antibody, anti-EC 3.1.3.48 antibody, anti-M-phase inducer phosphatase 3 antibody, anti-mitosis inducer CDC25 antibody, anti-phosphotyrosine phosphatase antibody
10 Results for "cdc25c" in Primary Antibodies
![Western Blot: CDC25C Antibody (3B11) [H00000995-M01] - Analysis of CDC25C expression in transfected 293T cell line by CDC25C monoclonal antibody (M01), clone 3B11.Lane 1: CDC25C transfected lysate(53.312 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CDC25C-Antibody-3B11-Western-Blot-H00000995-M01-img0012.jpg "Western Blot: CDC25C Antibody (3B11) [H00000995-M01] - Analysis of CDC25C expression in transfected 293T cell line by CDC25C monoclonal antibody (M01), clone 3B11.Lane 1: CDC25C transfected lysate(53.312 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: CDC25C Antibody (3B11) [H00000995-M01] - Analysis of monoclonal antibody to CDC25C on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 6 ug/ml.](http://images.novusbio.com/fullsize/CDC25C-Antibody-3B11-Immunohistochemistry-Paraffin-H00000995-M01-img0007.jpg)
![Western Blot: CDC25C Antibody (3B11) [H00000995-M01] - Analysis of CDC25C over-expressed 293 cell line, cotransfected with CDC25C Validated Chimera RNAi ( Cat # H00000995-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CDC25C monoclonal antibody (M01), clone 3B11 (Cat # H00000995-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/CDC25C-Antibody-3B11-Western-Blot-H00000995-M01-img0009.jpg)
![Western Blot: CDC25C Antibody (3B11) [H00000995-M01] - CDC25C monoclonal antibody (M01), clone 3B11 Analysis of CDC25C expression in HeLa.](http://images.novusbio.com/fullsize/CDC25C-Antibody-3B11-Western-Blot-H00000995-M01-img0011.jpg)
![Immunoprecipitation: CDC25C Antibody (3B11) [H00000995-M01] - Analysis of CDC25C transfected lysate using anti-CDC25C monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CDC25C MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/CDC25C-Antibody-3B11-Immunoprecipitation-H00000995-M01-img0008.jpg)
![Sandwich ELISA: CDC25C Antibody (3B11) [H00000995-M01] - Detection limit for recombinant GST tagged CDC25C is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CDC25C-Antibody-3B11-Sandwich-ELISA-H00000995-M01-img0010.jpg)
![Western Blot: CDC25C Antibody (JE59-53) [NBP3-32173] - Western blot analysis of CDC25C on different lysates with Rabbit anti-CDC25C antibody (NBP3-32173) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: Caco-2 cell lysate Lane 3: HUVEC cell lysate Lane 4: HT-29 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 58 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32173) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32173_rabbit-cdc25c-mab-je59-53-27820241631628.jpg "Western Blot: CDC25C Antibody (JE59-53) [NBP3-32173] - Western blot analysis of CDC25C on different lysates with Rabbit anti-CDC25C antibody (NBP3-32173) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: Caco-2 cell lysate Lane 3: HUVEC cell lysate Lane 4: HT-29 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 58 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32173) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CDC25C Antibody (JE59-53) [NBP3-32173] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-CDC25C antibody (NBP3-32173) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32173) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32173_rabbit-cdc25c-mab-je59-53-29820241420958.jpg)
![Immunocytochemistry/ Immunofluorescence: CDC25C Antibody (JE59-53) [NBP3-32173] - Immunocytochemistry analysis of HeLa cells labeling CDC25C with Rabbit anti-CDC25C antibody (NBP3-32173) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CDC25C antibody (NBP3-32173) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32173_rabbit-cdc25c-mab-je59-53-2920241023428.jpg)
![Flow Cytometry: CDC25C Antibody (JE59-53) [NBP3-32173] - Flow cytometric analysis of HeLa cells labeling CDC25C. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32173, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32173_rabbit-cdc25c-mab-je59-53-29202416453230.jpg)
![Western Blot: CDC25C Antibody [NBP2-15827] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CDC25C antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody](http://images.novusbio.com/fullsize/CDC25C-Antibody-Western-Blot-NBP2-15827-img0004.jpg "Western Blot: CDC25C Antibody [NBP2-15827] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CDC25C antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody")
![Immunocytochemistry/Immunofluorescence: Cdc25C Antibody [NBP2-15827] - Confocal immunofluorescence analysis of paraformaldehyde-fixed U2OS, using CDC25C antibody (Geen) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/Cdc25C-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15827-img0003.jpg)
![Immunohistochemistry-Paraffin: Cdc25C Antibody [NBP2-15827] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/Cdc25C-Antibody-Immunohistochemistry-Paraffin-NBP2-15827-img0002.jpg)
![Western Blot: Cdc25C Antibody [NBP2-15827] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE gel, diluted at 1:2000.](http://images.novusbio.com/fullsize/Cdc25C-Antibody-Western-Blot-NBP2-15827-img0001.jpg)
![Western Blot: CDC25C Antibody (3E6) [NBP3-15178] - Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, Raji whole cell lysate, 293 whole cell lysate. All lanes: CDC25C antibody at 1.65ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 54, 52, 49, 46 KDa. Observed band size: 60 KDa.](http://images.novusbio.com/fullsize/CDC25C-Antibody-3E6-Western-Blot-NBP3-15178-img0004.jpg "Western Blot: CDC25C Antibody (3E6) [NBP3-15178] - Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, Raji whole cell lysate, 293 whole cell lysate. All lanes: CDC25C antibody at 1.65ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 54, 52, 49, 46 KDa. Observed band size: 60 KDa.")
![Immunocytochemistry/Immunofluorescence: CDC25C Antibody (3E6) [NBP3-15178] - Immunofluorescence staining of Hela cells with at CDC25C antibody 1:55, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/CDC25C-Antibody-3E6-Immunocytochemistry-Immunofluorescence-NBP3-15178-img0001.jpg)
![Immunohistochemistry: CDC25C Antibody (3E6) [NBP3-15178] - IHC image of CDC25C antibody diluted at 1:165 and staining in paraffin-embedded human glioma cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/CDC25C-Antibody-3E6-Immunohistochemistry-NBP3-15178-img0003.jpg)
![Immunoprecipitation: CDC25C Antibody (3E6) [NBP3-15178] - Immunoprecipitating CDC25C in HEK293 whole cell lysate. Lane 1: Rabbit control IgG instead of CDC25C antibody in HEK293 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: CDC25C antibody (3ug) + HEK293 whole cell lysate (500ug). Lane 3: HEK293 whole cell lysate (20ug).](http://images.novusbio.com/fullsize/CDC25C-Antibody-3E6-Immunoprecipitation-NBP3-15178-img0002.jpg)
![Western Blot: CDC25C [p Ser216] Antibody [NB100-81841] - Analysis of extracts from 293 cells untreated or treated with serum using CDC25C [p Ser216] Antibody.](http://images.novusbio.com/fullsize/CDC25C-[p-Ser216]-Antibody-Western-Blot-NB100-81841-img0006.jpg "Western Blot: CDC25C [p Ser216] Antibody [NB100-81841] - Analysis of extracts from 293 cells untreated or treated with serum using CDC25C [p Ser216] Antibody.")
![Immunocytochemistry/Immunofluorescence: CDC25C [p Ser216] Antibody [NB100-81841] - Staining of methanol-fixed Hela cells using CDC25C [p Ser216] Antibody.](http://images.novusbio.com/fullsize/CDC25C-[p-Ser216]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-81841-img0004.jpg)
![Immunohistochemistry-Paraffin: CDC25C [p Ser216] Antibody [NB100-81841] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NB100-81841 (left) or the same antibody preincubated with blocking peptide(right).](http://images.novusbio.com/fullsize/CDC25C-[p-Ser216]-Antibody-Immunohistochemistry-Paraffin-NB100-81841-img0007.jpg)
![Western Blot: CDC25C Antibody (6R0I6) [NBP3-15437] - Western blot analysis of extracts of various cell lines, using CDC25C antibody (NBP3-15437) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/CDC25C-Antibody-6R0I6-Western-Blot-NBP3-15437-img0002.jpg "Western Blot: CDC25C Antibody (6R0I6) [NBP3-15437] - Western blot analysis of extracts of various cell lines, using CDC25C antibody (NBP3-15437) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunocytochemistry/ Immunofluorescence: CDC25C Antibody (6R0I6) [NBP3-15437] - Immunofluorescence analysis of HeLa cells using CDC25C Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15437_rabbit-cdc25c-mab-6r0i6-72202512435382.jpg)
![Western Blot: CDC25C Antibody (SR2051) [NBP3-21697] - Western blot analysis using NBP3-21697 in K562 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21697_rabbit-cdc25c-mab-sr2051-57202317161892.jpg "Western Blot: CDC25C Antibody (SR2051) [NBP3-21697] - Western blot analysis using NBP3-21697 in K562 cell lysate.")
![Immunocytochemistry/Immunofluorescence: CDC25C Antibody [NBP2-57705] - Staining of human cell line U-2 OS shows localization to nuclear speckles.](http://images.novusbio.com/fullsize/CDC25C-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57705-img0001.jpg "Immunocytochemistry/Immunofluorescence: CDC25C Antibody [NBP2-57705] - Staining of human cell line U-2 OS shows localization to nuclear speckles.")
![Western Blot: CDC25C [p Ser216] Antibody [NBP3-12879] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CDC25C (phospho Ser216) antibody (NBP3-12879) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CDC25C-[p-Ser216]-Antibody-Western-Blot-NBP3-12879-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: CDC25C [p Ser216] Antibody [NBP3-12879] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CDC25C (phospho Ser216) antibody (NBP3-12879) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")