CDC25C Antibody (3B11)

Images

 
Western Blot: CDC25C Antibody (3B11) [H00000995-M01] - Analysis of CDC25C expression in transfected 293T cell line by CDC25C monoclonal antibody (M01), clone 3B11.Lane 1: CDC25C transfected lysate(53.312 KDa).Lane 2: ...read more
Immunohistochemistry-Paraffin: CDC25C Antibody (3B11) [H00000995-M01] - Analysis of monoclonal antibody to CDC25C on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 6 ug/ml.
Western Blot: CDC25C Antibody (3B11) [H00000995-M01] - Analysis of CDC25C over-expressed 293 cell line, cotransfected with CDC25C Validated Chimera RNAi ( Cat # H00000995-R01V ) (Lane 2) or non-transfected control (Lane ...read more
Western Blot: CDC25C Antibody (3B11) [H00000995-M01] - CDC25C monoclonal antibody (M01), clone 3B11 Analysis of CDC25C expression in HeLa.
Immunoprecipitation: CDC25C Antibody (3B11) [H00000995-M01] - Analysis of CDC25C transfected lysate using anti-CDC25C monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CDC25C MaxPab rabbit ...read more
Sandwich ELISA: CDC25C Antibody (3B11) [H00000995-M01] - Detection limit for recombinant GST tagged CDC25C is approximately 0.1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IP, KD
Clone
3B11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

CDC25C Antibody (3B11) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
CDC25C (AAH19089, 21 a.a. ~ 130 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. FRSNQRKMLNLLLERDTSFTVCPDVPRTPVGKFLGDSANLSILSGGTPKCCLDLSNLSSGEITATQLTTSADLDETGHLDSSGLQEVHLAGMNHDQHLMKCSPAQLLCST
Marker
Mitosis Marker
Specificity
CDC25C - cell division cycle 25C
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CDC25C
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Knockdown Validated
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactivity against cell lysate, transfected lysate and recombinant protein for WB. It has been used for IHC-P, RNAi Validation and ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CDC25C Antibody (3B11)

  • CDC 25
  • CDC25
  • CDC25C
  • cell division cycle 25 homolog C (S. cerevisiae)
  • cell division cycle 25 homolog C (S. pombe)
  • cell division cycle 25C
  • Dual specificity phosphatase Cdc25C
  • EC 3.1.3.48
  • mitosis inducer CDC25
  • M-phase inducer phosphatase 3
  • phosphotyrosine phosphatase

Background

This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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H00000995-M01
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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CDC25C
Entrez
OMIM
Uniprot