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CD69 Antibody (H1.2F3) - Azide and BSA Free

 (3 Publications)
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Flow Cytometry: CD69 Antibody (H1.2F3) [NBP1-28011] - PMA and Ionomycin stimulated BALB/c mouse splenocytes were stained with Hamster Anti-Mouse CD69-PACBLU.
Flow Cytometry: CD69 Antibody (H1.2F3) [NBP1-28011] - PMA and Ionomycin stimulated BALB/c mouse splenocytes were stained with Hamster Anti-Mouse CD69-PACBLU.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
H1.2F3
Clonality
Monoclonal
Host
Armenian Hamster
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
0.5 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NBP1-28011AF405
Alexa Fluor 488
NBP1-28011AF488
Alexa Fluor 647
NBP1-28011AF647
Alexa Fluor 700
NBP1-28011AF700
Allophycocyanin
NBP1-28011APC
DyLight 350
NBP1-28011UV
DyLight 405
NBP1-28011V
DyLight 488
NBP1-28011G
DyLight 680
NBP1-28011FR
DyLight 755
NBP1-28011IR
FITC
NBP1-28012
PE
NBP1-28011PE
PerCP
NBP1-28011PCP
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CD69 Antibody (H1.2F3) - Azide and BSA Free Summary

Immunogen
Mouse dendritic epidermal cell line Y245
Specificity
Mouse CD69/Very Early Activation Antigen (VEA), Mr 85 kDa (unreduced)
Isotype
IgG1
Clonality
Monoclonal
Host
Armenian Hamster
Gene
CD69
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
Application Notes
Flow reactivity reported in (PMID: 29367423).
Publications
Read Publications using
NBP1-28011 in the following applications:

  • FLOW
    3 publications

Reactivity Notes

Mouse reactivity reported in (PMID: 29367423).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.1M BBS (pH 8.2)
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for CD69 Antibody (H1.2F3) - Azide and BSA Free

  • activation inducer molecule (AIM/CD69)
  • Activation inducer molecule
  • AIM
  • BL-AC/P26
  • CD69 antigen (p60, early T-cell activation antigen)
  • CD69 antigen
  • CD69 molecule
  • CD69
  • CLEC2CC-type lectin domain family 2 member C
  • C-type lectin domain family 2, member C
  • EA1
  • EA-1
  • early activation antigen CD69
  • early lymphocyte activation antigen
  • Early T-cell activation antigen p60
  • GP32/28
  • Leu23
  • Leukocyte surface antigen Leu-23
  • MLR-3
  • p60

Background

CD69, also known as very early activation (VEA) antigen, is a disulfide-linked transmembrane homodimer whose differentially glycosylated subunits range from 35-39 kDa. (1,2) It is a C-type lectin, most closely related to the NKR-P1 and Ly-49 NK cell-activation molecules. (3) CD69 is widely expressed on hematopoietic cells, including lymphocytes, neutrophils and eosinophils. (1-4) Although not detectable on resting lymphocytes, its expression is rapidly (within 2 hours) upregulated upon activation of T, B and NK cells, and neutrophils. (4) Constitutive expression of CD69 on subsets of thymocytes suggests that it may be involved in regulation of developmental events in addition to its role in activation of a variety of hematopoietic cells. (4,5) MAb H1.2F3 augments PMA-induced T-cell proliferation,1,6 and induces redirected lysis of Fc receptor-bearing target cells by NK cells. (7)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD69 Antibody (NBP1-28011)(3)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: FLOW.


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Bioinformatics

Gene Symbol CD69