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CD69 Antibody (8B6) - BSA Free

 (1 Publication)
 (2 Reviews)
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Western Blot: CD69 Antibody (8B6) [NBP1-51607] - Analysis using CD69 mouse mAb against, Jurkat (1), L1210 (2) and TPH-1 (3) cell lysate.
Western Blot: CD69 Antibody (8B6) [NBP1-51607] - Analysis using CD69 mouse mAb against, Jurkat (1), L1210 (2) and TPH-1 (3) cell lysate.
Immunohistochemistry-Paraffin: CD69 Antibody (8B6) [NBP1-51607] - Human tonsil tissue stained with CD69 antibody NBP1-51607. IHC-P image submitted by a verified customer review.
Flow Cytometry: CD69 Antibody (8B6) [NBP1-51607] - Analysis of Jurkat cells using CD69 mouse mAb (green) and negative control (purple).
Immunohistochemistry-Paraffin: CD69 Antibody (8B6) [NBP1-51607] - Analysis of human Tonsil tissues using anti-CD69 mouse mAb
Imaging Mass Cytometry: CD69 Antibody (8B6) [NBP1-51607] - Human bone marrow FFPE tissue section. DNA stained in blue, CD69 stained in white. IMC image submitted by a verified customer review.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC, MS
Clone
8B6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP1-51607-0.025ml
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CD69 Antibody (8B6) - BSA Free Summary

Immunogen
Purified recombinant fragment of human CD69 expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD69
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Imaging Mass Cytometry
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
Application Notes
CD69 antibody validated for IHC-P and Imaging Mass Cytometry from verified customer reviews.
Theoretical MW
22.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 4
using
NBP1-51607 in the following applications:
Publications
Read Publication using
NBP1-51607 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for CD69 Antibody (8B6) - BSA Free

  • activation inducer molecule (AIM/CD69)
  • Activation inducer molecule
  • AIM
  • BL-AC/P26
  • CD69 antigen (p60, early T-cell activation antigen)
  • CD69 antigen
  • CD69 molecule
  • CD69
  • CLEC2CC-type lectin domain family 2 member C
  • C-type lectin domain family 2, member C
  • EA1
  • EA-1
  • early activation antigen CD69
  • early lymphocyte activation antigen
  • Early T-cell activation antigen p60
  • GP32/28
  • Leu23
  • Leukocyte surface antigen Leu-23
  • MLR-3
  • p60

Background

CD69 is a 60 kD type II membrane protein composed of a 27/33 kD disulfide-linked homodimer, also known as Very Early Activation Antigen (VEA), AIM, EA1, MLR3 and gp34/28. It is expressed on a subset of thymocytes and platelets. CD69 is rapidly induced on activated T and B cells, neutrophils, and NK cells. It is a C-type lectin, closely related to the NKR-P1 and Ly-49 NK cell activation molecules. CD69 is involved in the early events of cell activation and thymocyte positive selection. The H1.2F3 antibody has been reported to augment T cell activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CD69 Antibody
(8B6) - BSA Free
NBP1-51607
CD69 Antibody (8B6) - BSA Free
Species: Hu
Applications: WB, ELISA, Flow, IHC, MS

Publications for CD69 Antibody (NBP1-51607)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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(1)
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Reviews for CD69 Antibody (NBP1-51607) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NBP1-51607:
Filter by Applications
IMC
(1)
IHC-P
(1)
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Filter by Species
Human
(2)
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Images Ratings Applications Species Date Details
  4
reviewed by:
Michela Colombo
 IMC Human 11/12/2021
View

Summary

ApplicationImaging Mass Cytometry
Sample Testedbone marrow
SpeciesHuman
Lot200417

Comments

Comments***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***
Immunohistochemistry-Paraffin CD69 NBP1-51607
Enlarge 		4
reviewed by:
Masahiro Hirata
 IHC-P Human 09/11/2020
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedhuman tonsil
SpeciesHuman
Lot150719

Comments

CommentsPretreatment: HIER, ER2(Leica)
Primary Ab: 1:1000
Detection system: BOND polymer refine detection kit (Leica)

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WB Video Protocol

FAQs for CD69 Antibody (NBP1-51607) (0)

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Secondary Antibodies

 

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Recent Reviews

4
5
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4
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1
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Imaging Mass Cytometry CD69 NBP1-51607
Enlarge
 Michela Colombo
11/12/2021
Application: IMC
Species: Human
read this review
Immunohistochemistry-Paraffin CD69 NBP1-51607
Enlarge
 Masahiro Hirata
09/11/2020
Application: IHC-P
Species: Human
read this review

Bioinformatics

Gene Symbol CD69