Home » CD69 » CD69 Antibodies » CD69 Antibody (310116) [Unconjugated]

CD69 Antibody (310116) [Unconjugated]

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CD69 was detected in immersion fixed mouse splenocytes using 10 µg/mL Rat Anti-Mouse CD69 Monoclonal Antibody (Catalog # MAB23861) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
CD69 was detected in immersion fixed mouse splenocytes using 10 µg/mL Rat Anti-Mouse CD69 Monoclonal Antibody (Catalog # MAB23861) for 3 hours at room temperature. Cells were stained with the NorthernLights™ ...read more
CD69 was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse CD69 Monoclonal Antibody (Catalog # MAB23861) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ICC/IF
Clone
310116
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB23861-SP
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CD69 Antibody (310116) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD69
Gly64-Arg199
Accession # P37217
Specificity
Detects mouse CD69 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant human CD69 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Gene
CD69
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publication using MAB23861.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD69 Antibody (310116) [Unconjugated]

  • activation inducer molecule (AIM/CD69)
  • Activation inducer molecule
  • AIM
  • BL-AC/P26
  • CD69 antigen (p60, early T-cell activation antigen)
  • CD69 antigen
  • CD69 molecule
  • CD69
  • CLEC2CC-type lectin domain family 2 member C
  • C-type lectin domain family 2, member C
  • EA1
  • EA-1
  • early activation antigen CD69
  • early lymphocyte activation antigen
  • Early T-cell activation antigen p60
  • GP32/28
  • Leu23
  • Leukocyte surface antigen Leu-23
  • MLR-3
  • p60

Background

CD69, also known as Leu 23, AIM, EA-1 and MLR-3, is a type II transmembrane glycoprotein and is expressed on activated T cells, B cells, NK cells, neutrophils and eosinophils, Langerhans cells and platelets.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CD69 Antibody
(310116) [Unconjugate
MAB23861
CD69 Antibody (310116) [Unconjugated]
Species: Mu
Applications: WB, ICC/IF

Publications for CD69 Antibody (MAB23861)(1)


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Bioinformatics

Gene Symbol CD69
Uniprot