CD63 Antibody (MX-49.129.5)

CD63 (cluster of differentiation 63), also known as lysosome associated membrane protein 3 (LAMP-3), is a membrane spanning glycoprotein with a molecular mass ranging from 30-60 kDa that was the first characterized member of the tetraspanin superfamily (1,2). CD63 is ubiquitously expressed and largely present on the cell surface in the endosomal system (1-3). More specifically, it is generally present in multivesicular bodies (MVBs), also called late endosomes, and lysosomes (1). The CD63 molecule is a total of 238 amino acids (aa), has four hydrophobic membrane-spanning regions and three N-glycosylation sites, and the encoded protein has a theoretical molecular weight of 25 kDa (2,3). CD63 both directly and indirectly interacts with other proteins including integrins, cell surface receptors, other tetraspanins, kinases, and adapter proteins, to name a few (1). CD63 is involved in many cell processes including cell survival and activation, cell adhesion, invasion, and migration (1). Additionally, CD63 has been shown to interact with tissue inhibitor of metalloproteinase 1 (TIMP-1), which originally thought to be an inhibitor of cancer progression has recently been shown to have cancer promoting properties as well (1,4). The CD63-TIMP-1 interaction has been shown to activate the PI3K/AKT pathway in lung adenocarcinoma cells and also promote survival and invasion of acute myeloid leukemia cells (1).CD63 is a useful flow cytometry marker for basophil granulocytes and can be used for the basophil activation test (BAT) to assess IgE-mediated allergy response (5). As CD63 was initially discovered on activated blood platelets and is a lysosomal-associated protein it makes sense it would be involved in platelet or lysosomal-related disorders (1,2). More precisely, CD63 is associated with Hermansky-Pudlak syndrome, a disease characterized by albinism and platelet storage pool deficiency (6).

References

1. Pols, M. S., & Klumperman, J. (2009). Trafficking and function of the tetraspanin CD63. Experimental cell research. https://doi.org/10.1016/j.yexcr.2008.09.020

2. Metzelaar, M. J., Wijngaard, P. L., Peters, P. J., Sixma, J. J., Nieuwenhuis, H. K., & Clevers, H. C. (1991). CD63 antigen. A novel lysosomal membrane glycoprotein, cloned by a screening procedure for intracellular antigens in eukaryotic cells. The Journal of biological chemistry.

3. Horejsi, V., & Vlcek, C. (1991). Novel structurally distinct family of leucocyte surface glycoproteins including CD9, CD37, CD53 and CD63. FEBS letters. https://doi.org/10.1016/0014-5793(91)80988-f

4. Eckfeld, C., HauBler, D., Schoeps, B., Hermann, C. D., & Kruger, A. (2019). Functional disparities within the TIMP family in cancer: hints from molecular divergence. Cancer metastasis reviews. https://doi.org/10.1007/s10555-019-09812-6

5. Hoffmann, H. J., Santos, A. F., Mayorga, C., Nopp, A., Eberlein, B., Ferrer, M., Rouzaire, P., Ebo, D. G., Sabato, V., Sanz, M. L., Pecaric-Petkovic, T., Patil, S. U., Hausmann, O. V., Shreffler, W. G., Korosec, P., & Knol, E. F. (2015). The clinical utility of basophil activation testing in diagnosis and monitoring of allergic disease. Allergy. https://doi.org/10.1111/all.12698

6. Dell'Angelica, E. C., Shotelersuk, V., Aguilar, R. C., Gahl, W. A., & Bonifacino, J. S. (1999). Altered trafficking of lysosomal proteins in Hermansky-Pudlak syndrome due to mutations in the beta 3A subunit of the AP-3 adaptor. Molecular cell. https://doi.org/10.1016/s1097-2765(00)80170-7

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 7 applications: FLOW, ICC/IF, IF/IHC, IHC-P, Immunohistochemistry-Paraffin, WB, Western Blot.

Showing Publications 1 - 10 of 16. Show All 16 Publications. Collapse Publications.

Publications using NBP2-32830	Applications	Species
Yiwei Ai, Chenxu Guo, Marta Garcia-Contreras, Laura S. Sánchez B., Andras Saftics, Oluwapelumi Shodubi, Shankar Raghunandan, Junhao Xu, Shang Jui Tsai, Yi Dong, Rong Li, Tijana Jovanovic-Talisman, Stephen J. Gould Endocytosis blocks the vesicular secretion of exosome marker proteins Science Advances 2024-05-10 [PMID: 38718108]
Kammala AK, Mosebarger A, Radnaa E et al. Extracellular Vesicles-mediated recombinant IL-10 protects against ascending infection-associated preterm birth by reducing fetal inflammatory response Frontiers in Immunology 2023-08-04 [PMID: 37600782] (Western Blot)	Western Blot
Plebanek, MP;Xue, Y;Nguyen, YV;DeVito, NC;Wang, X;Holtzhausen, A;Beasley, GM;Yarla, N;Thievanthiran, B;Hanks, BA; A SREBF2-dependent gene program drives an immunotolerant dendritic cell population during cancer progression bioRxiv : the preprint server for biology 2023-04-28 [PMID: 37162965] (Immunohistochemistry-Paraffin, Mouse)	Immunohistochemistry-Paraffin	Mouse
Guo C, Tsai SJ, Ai Y et al. The D614G mutation redirects SARS-CoV-2 spike to lysosomes and suppresses deleterious traits of the furin cleavage site insertion mutation Science advances 2022-12-23 [PMID: 36563151]
Hu G, Li G, Huang D et al. Renomedullary exosomes produce antihypertensive effects in reversible two-kidney one-clip renovascular hypertensive mice Biochemical pharmacology 2022-08-30 [PMID: 36055382] (IHC-P, Mouse)	IHC-P	Mouse
Tsai SJ, Ai Y, Guo C, Gould SJ Degron-tagging of BleoR and other antibiotic-resistance genes selects for higher expression of linked transgenes and improved exosome engineering The Journal of biological chemistry 2022-03-18 [PMID: 35314197] (WB)	WB
Tantengco OAG, Radnaa E, Shahin H et al. Cross talk: Trafficking and functional impact of maternal exosomes at the Feto-maternal Interface under normal and pathologic states Biology of reproduction 2021-09-23 [PMID: 34554204] (WB, Human)	WB	Human
Zou Y, Bhat Om, Yuan X Et Al. Release and Actions of Inflammatory Exosomes in Pulmonary Emphysema: Potential Therapeutic Target of Acupuncture Journal of Inflammation Research 2021-07-01 [PMID: 34335040] (IF/IHC)	IF/IHC
Radnaa E, Richardson L, Sheller-Miller S et al. Extracellular vesicle mediated feto-maternal HMGB1 signaling induces preterm birth Lab on a Chip 2021-03-29 [PMID: 34008619]
Shahin HI, Radnaa E, Tantengco OAG et al. Microvesicles and Exosomes Released by Amnion Epithelial Cells Under Oxidative Stress Cause Inflammatory Changes in Uterine Cells Biology of reproduction 2021-05-07 [PMID: 33962471] (WB, Human)	WB	Human
Han C, Kang H, Yi J et al. Single-vesicle imaging and co-localization analysis for tetraspanin profiling of individual extracellular vesicles Extracell Vesicles 2021-01-10 [PMID: 33456726]
Kumar A, Sundaram K, Mu J, et al. High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance Nature communications 2021-01-11 [PMID: 33431899] (Mouse)		Mouse
Guo C, Tsai SJ, Ai Y et al. The D614G Mutation Enhances the Lysosomal Trafficking of SARS-CoV-2 Spike bioRxiv : the preprint server for biology 2020-12-09 [PMID: 33330866] (ICC/IF, Human)	ICC/IF	Human
Sheller-Miller S, Trivedi J, Yellon SM, Menon R. Exosomes Cause Preterm Birth in Mice: Evidence for Paracrine Signaling in Pregnancy. Sci Rep 2019-01-24 [PMID: 30679631] (FLOW, Human)	FLOW	Human
Yuan, P; SOX10 downregulation in tumor cells affects extracellular vesicle production and function in the tumor microenvironment Thesis
Tantengco O, Richardson L, Radnaa E et al. Exosomes from Ureaplasma parvum-infected ectocervical epithelial cells promote feto-maternal interface inflammation but are insufficient to cause preterm delivery Frontiers in Cell and Developmental Biology 2022-08-15 [PMID: 36046342] (WB, Human)	WB	Human
Show All 16 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	5 reviewed by: Verified Customer	WB	Human	10/20/2022	View Summary Application Western Blot Sample Tested MDA-MB-231 Species Human Lot 967-2P220115
	5 reviewed by: Verified Customer	WB	Human	08/03/2022	View Summary Application Western Blot Sample Tested 293T cell lysate Species Human Lot 967-2P191216 Comments Comments 1:500 in TBST

Array NBP2-32830

• CD63 Antibodies
• CD63 ELISA Kit
• CD63 Lysate
• CD63 Proteins
• CD63 Proteins and Enzyme