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CD63 Antibody (2585J) [Unconjugated]

 (3 Publications)
 
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Western blot shows lysates of human platelets. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human CD63 Monoclonal Antibody (Catalog # MAB50482) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for CD63 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human platelets. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human CD63 Monoclonal Antibody (Catalog # MAB50482) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody ...read more
CD63 was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Rabbit Anti-Human CD63 Monoclonal Antibody (Catalog # MAB50482) at 0.3 µg/mL for 1 hour at room temperature followed by ...read more
Human PBMC were stained with Rabbit Anti-Human CD63 Monoclonal Antibody (Catalog # MAB50482, filled histogram) or isotype control antibody (Catalog # MAB1050, open histogram), followed by Phycoerythrin-conjugated ...read more
Simple Western lane view shows lysates of human platelets, loaded at 0.2 mg/mL. A specific band was detected for CD63 at approximately 59 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Human CD63 Monoclonal Antibody ...read more
PBMC monocytes were stained with Rabbit Anti-Human CD63 Monoclonal Antibody (Catalog # MAB50482, filled histogram) or isotype control antibody (Catalog # MAB1050, open histogram) followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, Flow, IHC, CyTOF-ready
Clone
2585J
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC50482U-100UG
Alexa Fluor 405
IC50482V-100UG
Alexa Fluor 488
IC50482G-100UG
Alexa Fluor 532
FAB50482X
Alexa Fluor 594
IC50482T-100UG
Alexa Fluor 647
IC50482R-100UG
Alexa Fluor 700
IC50482N-100UG
Alexa Fluor 750
IC50482S-100UG
Allophycocyanin
FAB50482A
Allophycocyanin/Cy7
FAB50482APCCY7
Biotin
FAB50482B
DyLight 350
FAB50482D
DyLight 405
FAB50482E
DyLight 488
FAB50482K
DyLight 550
FAB50482L
DyLight 594
FAB50482M
DyLight 650
FAB50482W
DyLight 680
FAB50482Y
DyLight 755
FAB50482Z
FITC
FAB50482F
HRP
FAB50482H
Janelia Fluor 549
FAB50482I
Janelia Fluor 646
FAB50482J
PE
FAB50482P
PE/Atto594
FAB50482PEATT594
PE/Cy5.5
FAB50482PECY55
PE/Cy7
FAB50482PECY7
PerCP
FAB50482C
mFluor Violet 450 SE
FAB50482MFV450
mFluor Violet 500 SE
FAB50482MFV500
mFluor Violet 610 SE
FAB50482MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB50482-SP
Carrier Free
IC50482V-100UG
Carrier Free
IC50482U-100UG
Carrier Free
IC50482T-100UG
Carrier Free
IC50482S-100UG
Carrier Free
IC50482R-100UG
Carrier Free
IC50482N-100UG
Carrier Free
IC50482G-100UG
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CD63 Antibody (2585J) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Chinese Hamster Ovary cell line CHO-derived human CD63
Ala103-Val203
Accession # P08962-1
Specificity
Detects human CD63 in direct ELISAs.
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Purity Statement
Protein A or G purified from cell culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 0.3-25 ug/mL
  • Simple Western 20 ug/mL
  • Western Blot 0.5 ug/mL
Publications
Read Publications using
MAB50482 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD63 Antibody (2585J) [Unconjugated]

  • CD_antigen: CD63
  • CD63 antigen (melanoma 1 antigen)
  • CD63 antigen
  • CD63 molecule
  • CD63
  • Granulophysin
  • Lamp-3
  • Lysosomal-associated membrane protein 3
  • lysosome-associated membrane glycoprotein 3
  • ME491
  • melanoma 1 antigen
  • Melanoma-associated antigen ME491
  • melanoma-associated antigen MLA1
  • MLA1
  • Ocular melanoma-associated antigen
  • OMA81H
  • tetraspanin-30
  • Tspan30
  • tspan-30

Background

CD63, also known as LAMP-3 or ME491 (melanoma-associated antigen), is a 30-60 kDa member of the tetraspanin superfamily of protein trafficking proteins. CD63 is ubiquitously expressed and found in late endocytic vesicles, but following cell activation is also present on the plasma membrane. Interaction of CD63 with other membrane proteins or adaptors regulates cell activities such as adhesion, migration and degranulation. Extracellular regions of human CD63 share 67% and 65% amino acid sequence identity with mouse and rat CD63, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD63 Antibody (MAB50482)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: Cell Isolation, SDS-Page, Western Blot.


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Cell Isolation
(1)
SDS-Page
(1)
Western Blot
(3)
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Human
(3)
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