Reactivity | HuSpecies Glossary |
Applications | WB, Flow, ICC/IF, IHC |
Clone | YTH53.1 |
Clonality | Monoclonal |
Host | Rat |
Conjugate | CoraFluor 1 |
Description | CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays. |
Additional Information | Clone YTH53.1 was used by HLDA to establish CD designation. |
Immunogen | Human Peripheral Blood T cells |
Localization | Cell membrane; lipid anchor; GPI anchor. |
Specificity | NB100-65537 recognizes the human CD59 cell surface antigen, an 18-20kD GPI linked glycoprotein broadly expressed by human leucocytes and erythrocytes. Clone YTH53.1 has been shown to block the normal function of CD59. Removal of sodium azide is recommended prior to use in functional assays. CD59 is expressed by erythrocytes, and therefore staining in whole blood systems requires a higher concentration of antibody. |
Isotype | IgG2b |
Clonality | Monoclonal |
Host | Rat |
Gene | CD59 |
Purity | Protein G purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | Optimal dilution of this antibody should be experimentally determined. |
Storage | Store at 4C in the dark. Do not freeze. |
Buffer | PBS |
Preservative | No Preservative |
Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for CD59 Antibody (NB100-65537CL1)Find related products by research area.
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Gene Symbol | CD59 |