![Immunohistochemistry-Paraffin: CD59 Antibody (MACIF/629) - IHC-Prediluted [NBP2-48466] - Formalin-fixed, paraffin-embedded human Tongue stained with CD59 Mouse Monoclonal Antibody (MACIF/629).](http://images.novusbio.com/fullsize/CD59-Antibody-MACIF-629-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48466-img0002.jpg "Immunohistochemistry-Paraffin: CD59 Antibody (MACIF/629) - IHC-Prediluted [NBP2-48466] - Formalin-fixed, paraffin-embedded human Tongue stained with CD59 Mouse Monoclonal Antibody (MACIF/629).")
| Reactivity | HuSpecies Glossary |
| Applications | IHC |
| Clone | MACIF/629 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | IHC-Prediluted |
| Description | The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining. |
| Immunogen | Human K562 tumor cells |
| Localization | Cell Surface |
| Specificity | Reacts with human CD59, a 20kDa glycosyl phosphatidyl-inositol (GPI)-anchored cell surface protein. CD59 regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. CD59 is widely distributed on cells in all tissues. It inhibits formation of MAC, thus protecting cells from complement-mediated lysis. The expression of CD59 on erythrocytes is important for their survival. Genetic defects in GPI-anchor attachment, that cause a reduction or loss of CD59 and CD55 on erythrocytes produce the symptoms of the disease paroxysmal hemoglobinuria (PNH). This monoclonal antibody is useful for study on GPI-anchored proteins, PNH and CD59 functions. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CD59 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 20 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for CD59 Antibody (NBP2-48466)Find related products by research area.
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| Gene Symbol | CD59 |
![Immunohistochemistry-Paraffin: CD59 Antibody (MACIF/629) - IHC-Prediluted [NBP2-48466] - Formalin-fixed, paraffin-embedded human Tongue stained with CD59 Mouse Monoclonal Antibody (MACIF/629).](http://images.novusbio.com/fullsize/CD59-Antibody-MACIF-629-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48466-img0001.jpg "Immunohistochemistry-Paraffin: CD59 Antibody (MACIF/629) - IHC-Prediluted [NBP2-48466] - Formalin-fixed, paraffin-embedded human Tongue stained with CD59 Mouse Monoclonal Antibody (MACIF/629).")
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