![Western Blot: CD59 Antibody (8D2B8) [NBP2-37436] - Western blot analysis using CD59 mAb against HEK293 (1) and CD59 (AA: 31-111)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/CD59-Antibody-8D2B8-Western-Blot-NBP2-37436-img0003.jpg "Western Blot: CD59 Antibody (8D2B8) [NBP2-37436] - Western blot analysis using CD59 mAb against HEK293 (1) and CD59 (AA: 31-111)-hIgGFc transfected HEK293 (2) cell lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | 8D2B8 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Purified recombinant fragment of human CD59 (AA: 31-111) expressed in E. Coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CD59 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody is Cytof ready. |
| Theoretical MW | 14.2 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for CD59 Antibody (NBP2-37436)Find related products by research area.
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| Gene Symbol | CD59 |
![Immunocytochemistry/Immunofluorescence: CD59 Antibody (8D2B8) [NBP2-37436] - Immunofluorescence analysis of Hela cells using CD59 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/CD59-Antibody-8D2B8-Immunocytochemistry-Immunofluorescence-NBP2-37436-img0005.jpg "Immunocytochemistry/Immunofluorescence: CD59 Antibody (8D2B8) [NBP2-37436] - Immunofluorescence analysis of Hela cells using CD59 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.")
![Immunohistochemistry: CD59 Antibody (8D2B8) [NBP2-37436] - Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using CD59 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CD59-Antibody-8D2B8-Immunohistochemistry-NBP2-37436-img0008.jpg "Immunohistochemistry: CD59 Antibody (8D2B8) [NBP2-37436] - Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using CD59 mouse mAb with DAB staining.")
![Flow Cytometry: CD59 Antibody (8D2B8) [NBP2-37436] - Flow cytometric analysis of HeLa cells using CD59 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/CD59-Antibody-8D2B8-Flow-Cytometry-NBP2-37436-img0006.jpg "Flow Cytometry: CD59 Antibody (8D2B8) [NBP2-37436] - Flow cytometric analysis of HeLa cells using CD59 mouse mAb (green) and negative control (red).")
![Western Blot: CD59 Antibody (8D2B8) [NBP2-37436] - Western blot analysis using CD59 mAb against human CD59 recombinant protein. (Expected MW is 34.7 kDa)](http://images.novusbio.com/fullsize/CD59-Antibody-8D2B8-Western-Blot-NBP2-37436-img0002.jpg "Western Blot: CD59 Antibody (8D2B8) [NBP2-37436] - Western blot analysis using CD59 mAb against human CD59 recombinant protein. (Expected MW is 34.7 kDa)")
![Immunocytochemistry/Immunofluorescence: CD59 Antibody (8D2B8) [NBP2-37436] - Immunofluorescence analysis of MCF-7 cells using CD59 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/CD59-Antibody-8D2B8-Immunocytochemistry-Immunofluorescence-NBP2-37436-img0004.jpg "Immunocytochemistry/Immunofluorescence: CD59 Antibody (8D2B8) [NBP2-37436] - Immunofluorescence analysis of MCF-7 cells using CD59 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.")
![Immunohistochemistry: CD59 Antibody (8D2B8) [NBP2-37436] - Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using CD59 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CD59-Antibody-8D2B8-Immunohistochemistry-NBP2-37436-img0007.jpg "Immunohistochemistry: CD59 Antibody (8D2B8) [NBP2-37436] - Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using CD59 mouse mAb with DAB staining.")
![ELISA: CD59 Antibody (8D2B8) [NBP2-37436] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/CD59-Antibody-8D2B8-ELISA-NBP2-37436-img0001.jpg "ELISA: CD59 Antibody (8D2B8) [NBP2-37436] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")